CPLM-004468

Genbank Nucleotide ID

Methylated-DNA--protein-cysteine methyltransferase

Protein Synonyms/Alias

6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Homo sapiens (Human)

Position	Peptide	Type	References
8	MDKDCEMKRTTLDSP	ubiquitination	[1]
32	EQGLHEIKLLGKGTS	ubiquitination	[1]
101	FTRQVLWKLLKVVKF	ubiquitination	[1]
104	QVLWKLLKVVKFGEV	ubiquitination	[1]
125	AALAGNPKAARAVGG	ubiquitination	[1]
165	YSGGLAVKEWLLAHE	acetylation	[2]
165	YSGGLAVKEWLLAHE	ubiquitination	[1]
178	HEGHRLGKPGLGGSS	ubiquitination	[1, 2, 3, 4]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]

Functional Description

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

ACT_SITE 145 145 Nucleophile; methyl group acceptor.
METAL 5 5 Zinc.
METAL 24 24 Zinc.
METAL 29 29 Zinc.
METAL 85 85 Zinc.
BINDING 95 95 DNA; via amide nitrogen.
BINDING 114 114 DNA.
BINDING 115 115 DNA; via amide nitrogen.
BINDING 123 123 DNA.
BINDING 128 128 DNA.
BINDING 151 151 DNA; via amide nitrogen.

3D-structure; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; DNA-binding; Metal-binding; Methyltransferase; Nucleus; Polymorphism; Reference proteome; Transferase; Zinc.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005654; C:nucleoplasm; TAS:Reactome.
GO:0005509; F:calcium ion binding; IEA:Compara.
GO:0003677; F:DNA binding; TAS:ProtInc.
GO:0009008; F:DNA-methyltransferase activity; TAS:ProtInc.
GO:0003908; F:methylated-DNA-[protein]-cysteine S-methyltransferase activity; IEA:EC.
GO:0071479; P:cellular response to ionizing radiation; IEA:Compara.
GO:0071407; P:cellular response to organic cyclic compound; IEA:Compara.
GO:0034599; P:cellular response to oxidative stress; IEA:Compara.
GO:0006307; P:DNA dealkylation involved in DNA repair; IEA:Compara.
GO:0006266; P:DNA ligation; TAS:ProtInc.
GO:0006281; P:DNA repair; TAS:Reactome.
GO:0060644; P:mammary gland epithelial cell differentiation; IEA:Compara.
GO:0060548; P:negative regulation of cell death; IEA:Compara.
GO:0045739; P:positive regulation of DNA repair; IEA:Compara.
GO:0043281; P:regulation of cysteine-type endopeptidase activity involved in apoptotic process; IEA:Compara.
GO:0042493; P:response to drug; IEA:Compara.
GO:0045471; P:response to ethanol; IEA:Compara.
GO:0051593; P:response to folic acid; IEA:Compara.
GO:0009636; P:response to toxic substance; IEA:Compara.

IPR001497; MethylDNA_cys_MeTrfase_AS.
IPR014048; MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332; MethylG_MeTrfase.
IPR011991; WHTH_DNA-bd_dom.

PF01035; DNA_binding_1
PF02870; Methyltransf_1N