CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004742
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 General amino-acid permease GAP1 
Protein Synonyms/Alias
  
Gene Name
 GAP1 
Gene Synonyms/Alias
 YKR039W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
76PNLSEAEKVAIITAQubiquitination[1, 2, 3, 4, 5]
Reference
 [1] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [2] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538]
 [3] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [4] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [5] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Permease for various amino acids as well as for GABA. Can also transport L-cysteine and beta-alanine. 
Sequence Annotation
 CROSSLNK 76 76 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Amino-acid transport; Complete proteome; Isopeptide bond; Membrane; Reference proteome; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 602 AA 
Protein Sequence
MSNTSSYEKN NPDNLKHNGI TIDSEFLTQE PITIPSNGSA VSIDETGSGS KWQDFKDSFK 60
RVKPIEVDPN LSEAEKVAII TAQTPLKHHL KNRHLQMIAI GGAIGTGLLV GSGTALRTGG 120
PASLLIGWGS TGTMIYAMVM ALGELAVIFP ISGGFTTYAT RFIDESFGYA NNFNYMLQWL 180
VVLPLEIVSA SITVNFWGTD PKYRDGFVAL FWLAIVIINM FGVKGYGEAE FVFSFIKVIT 240
VVGFIILGII LNCGGGPTGG YIGGKYWHDP GAFAGDTPGA KFKGVCSVFV TAAFSFAGSE 300
LVGLAASESV EPRKSVPKAA KQVFWRITLF YILSLLMIGL LVPYNDKSLI GASSVDAAAS 360
PFVIAIKTHG IKGLPSVVNV VILIAVLSVG NSAIYACSRT MVALAEQRFL PEIFSYVDRK 420
GRPLVGIAVT SAFGLIAFVA ASKKEGEVFN WLLALSGLSS LFTWGGICIC HIRFRKALAA 480
QGRGLDELSF KSPTGVWGSY WGLFMVIIMF IAQFYVAVFP VGDSPSAEGF FEAYLSFPLV 540
MVMYIGHKIY KRNWKLFIPA EKMDIDTGRR EVDLDLLKQE IAEEKAIMAT KPRWYRIWNF 600
WC 602 
Gene Ontology
 GO:0030134; C:ER to Golgi transport vesicle; IDA:SGD.
 GO:0000328; C:fungal-type vacuole lumen; IDA:SGD.
 GO:0005887; C:integral to plasma membrane; IDA:SGD.
 GO:0005771; C:multivesicular body; IMP:SGD.
 GO:0015193; F:L-proline transmembrane transporter activity; IGI:SGD.
 GO:0015203; F:polyamine transmembrane transporter activity; IMP:SGD. 
Interpro
 IPR004841; AA-permease_dom.
 IPR002293; AA/rel_permease1.
 IPR004762; Amino_acid_permease_fungi.
 IPR004840; Amoino_acid_permease_CS. 
Pfam
 PF00324; AA_permease 
SMART
  
PROSITE
 PS00218; AMINO_ACID_PERMEASE_1 
PRINTS