UniProt Accession

 FBSP1_HUMAN; P0C2W1; A6NF90; D3DXB5 

Genbank Protein ID

 AC092933; CH471191; CH471191; AK025697 

Genbank Nucleotide ID

 EAW53643.1; EAW53644.1 

Protein Name

 F-box/SPRY domain-containing protein 1 

Protein Synonyms/Alias

 F-box only protein 45; hFbxo45 

Gene Name

 FBXO45 

Gene Synonyms/Alias

 FBX45 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
64	RSCALVCKHWYRCLH	ubiquitination	[1]
104	LCNLPSYKAKIRAFQ	ubiquitination	[1, 2]
106	NLPSYKAKIRAFQHA	ubiquitination	[1]
127	SRNVYIKKNGFTLHR	ubiquitination	[1]
147	STDGARTKIGFSEGR	ubiquitination	[1, 3]
239	VILDMEDKTLAFERG	ubiquitination	[1, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Component of E3 ubiquitin ligase complexes. Required for normal neuromuscular synaptogenesis, axon pathfinding and neuronal migration (By similarity). Plays a role in the regulation of neurotransmission at mature neurons (By similarity). May controls synaptic activity by controlling UNC13A via ubiquitin dependent pathway (By similarity). Specifically recognizes TP73, promoting its ubiquitination and degradation. 

Sequence Annotation

 DOMAIN 33 82 F-box.
 DOMAIN 92 284 B30.2/SPRY.
 MOD_RES 2 2 N-acetylalanine.  

Keyword

 Acetylation; Cell junction; Cell membrane; Complete proteome; Developmental protein; Membrane; Neurogenesis; Postsynaptic cell membrane; Reference proteome; Synapse; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 286 AA 

Protein Sequence

Gene Ontology

 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
 GO:0014069; C:postsynaptic density; IEA:Compara.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
 GO:0042734; C:presynaptic membrane; IEA:UniProtKB-SubCell.
 GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
 GO:0006974; P:response to DNA damage stimulus; IDA:UniProtKB. 

Interpro

 IPR001870; B30.2/SPRY.
 IPR008985; ConA-like_lec_gl_sf.
 IPR001810; F-box_dom_cyclin-like.
 IPR018355; SPla/RYanodine_receptor_subgr.
 IPR003877; SPRY_rcpt. 

Pfam

 PF00622; SPRY 

SMART

 SM00256; FBOX
 SM00449; SPRY 

PROSITE

 PS50188; B302_SPRY
 PS50181; FBOX 

PRINTS