UniProt Accession

 ALF_YEAST; P14540; D6VXM7 

Genbank Protein ID

 X15003; Z28060; X75781; M32026; BK006944 

Genbank Nucleotide ID

 CAA33111.1; CAA81897.1; CAA53412.1; DAA09097.1 

Protein Name

 Fructose-bisphosphate aldolase 

Protein Synonyms/Alias

 FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase 

Gene Name

 FBA1 

Gene Synonyms/Alias

 YKL060C; YKL320 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
27	HNLFTYAKEHKFAIP	acetylation	[1]
27	HNLFTYAKEHKFAIP	ubiquitination	[2]
30	FTYAKEHKFAIPAIN	acetylation	[1]
30	FTYAKEHKFAIPAIN	ubiquitination	[3]
55	LEAARDSKSPIILQT	acetylation	[1]
73	GAAYFAGKGISNEGQ	acetylation	[1]
73	GAAYFAGKGISNEGQ	ubiquitination	[2, 3]
85	EGQNASIKGAIAAAH	acetylation	[1]
85	EGQNASIKGAIAAAH	ubiquitination	[2, 3]
114	LHSDHCAKKLLPWFD	acetylation	[1]
199	DKEDLYTKPEQVYNV	acetylation	[1]
246	EILAEHQKYTREQVG	ubiquitination	[3]
255	TREQVGCKEEKPLFL	ubiquitination	[2, 3]
258	QVGCKEEKPLFLVFH	acetylation	[1]
258	QVGCKEEKPLFLVFH	ubiquitination	[2, 4]
307	IRDYVLNKKDYIMSP	acetylation	[1]
307	IRDYVLNKKDYIMSP	ubiquitination	[3]
308	RDYVLNKKDYIMSPV	acetylation	[1]
308	RDYVLNKKDYIMSPV	ubiquitination	[2]
323	GNPEGPEKPNKKFFD	acetylation	[1]
323	GNPEGPEKPNKKFFD	ubiquitination	[2]
326	EGPEKPNKKFFDPRV	acetylation	[1]
326	EGPEKPNKKFFDPRV	ubiquitination	[2, 3]
327	GPEKPNKKFFDPRVW	acetylation	[1]
345	GEKTMGAKITKSLET	ubiquitination	[3]
348	TMGAKITKSLETFRT	acetylation	[1]
348	TMGAKITKSLETFRT	ubiquitination	[3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [4] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

 Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis (By similarity). 

Sequence Annotation

 REGION 266 268 Dihydroxyacetone phosphate binding (By
 REGION 287 290 Dihydroxyacetone phosphate binding (By
 ACT_SITE 110 110 Proton donor (By similarity).
 METAL 111 111 Zinc 1; catalytic (By similarity).
 METAL 145 145 Zinc 2 (By similarity).
 METAL 175 175 Zinc 2 (By similarity).
 METAL 227 227 Zinc 1; catalytic (By similarity).
 METAL 265 265 Zinc 1; catalytic (By similarity).
 BINDING 63 63 Glyceraldehyde 3-phosphate (By
 BINDING 228 228 Dihydroxyacetone phosphate; via amide
 MOD_RES 11 11 Phosphothreonine.
 MOD_RES 56 56 Phosphoserine.
 MOD_RES 63 63 Phosphoserine.
 MOD_RES 76 76 Phosphoserine.
 MOD_RES 83 83 Phosphoserine.
 MOD_RES 96 96 Phosphoserine.
 MOD_RES 147 147 Phosphoserine.
 MOD_RES 150 150 Phosphothreonine.
 MOD_RES 179 179 Phosphothreonine.
 MOD_RES 290 290 Phosphothreonine.
 MOD_RES 310 310 Phosphotyrosine.
 MOD_RES 313 313 Phosphoserine.  

Keyword

 Complete proteome; Direct protein sequencing; Glycolysis; Lyase; Metal-binding; Phosphoprotein; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 359 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0004332; F:fructose-bisphosphate aldolase activity; IMP:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006094; P:gluconeogenesis; IMP:SGD.
 GO:0006096; P:glycolysis; IMP:SGD. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR006411; Fruct_bisP_bact.
 IPR000771; Ketose_bisP_aldolase_II. 

Pfam

 PF01116; F_bP_aldolase 

SMART

  

PROSITE

 PS00602; ALDOLASE_CLASS_II_1
 PS00806; ALDOLASE_CLASS_II_2 

PRINTS