UniProt Accession

 SHPS1_HUMAN; P78324; A2A2E1; A8K411; B2R6C3; O00683; O43799; Q8N517; Q8TAL8; Q9H0Z2; Q9UDX2; Q9UIJ6; Q9Y4U9 

Genbank Protein ID

 D86043; Y10375; AB023430; AK290776; AK312521; AL034562; AL034562; AL117335; AL117335; AL117335; AL034562; BC026692; BC033092; BC038510; BC075849; Y11047 

Genbank Nucleotide ID

 BAA12974.1; CAA71403.1; BAA87929.1; BAF83465.1; BAG35420.1; CAB38874.2; CAM28287.1; CAM28287.1; CAC12723.2; CAM28335.1; CAM28335.1; AAH26692.1; AAH33092.1; AAH38510.1; AAH75849.1; CAA71944.1 

Protein Name

 Tyrosine-protein phosphatase non-receptor type substrate 1 

Protein Synonyms/Alias

 SHP substrate 1; SHPS-1; Brain Ig-like molecule with tyrosine-based activation motifs; Bit; CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MyD-1 antigen; Signal-regulatory protein alpha-1; Sirp-alpha-1; Signal-regulatory protein alpha-2; Sirp-alpha-2; Signal-regulatory protein alpha-3; Sirp-alpha-3; p84; CD172a 

Gene Name

 SIRPA 

Gene Synonyms/Alias

 BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
183	SPRDITLKWFKNGNE	ubiquitination	[1]
415	TRLHEPEKNAREITQ	ubiquitination	[2]
436	YADLNLPKGKKPAPQ	ubiquitination	[3]
483	VHLNRTPKQPAPKPE	acetylation	[4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. 

Sequence Annotation

 DOMAIN 32 137 Ig-like V-type.
 DOMAIN 148 247 Ig-like C1-type 1.
 DOMAIN 254 348 Ig-like C1-type 2.
 MOTIF 429 432 SH2-binding (Potential).
 MOTIF 439 444 SH3-binding (Potential).
 MOTIF 453 456 SH2-binding (Potential).
 MOTIF 470 473 SH2-binding (Potential).
 MOTIF 496 499 SH2-binding (Potential).
 MOD_RES 429 429 Phosphotyrosine; by Tyr-kinases
 MOD_RES 453 453 Phosphotyrosine; by Tyr-kinases
 MOD_RES 470 470 Phosphotyrosine; by Tyr-kinases (By
 MOD_RES 496 496 Phosphotyrosine; by Tyr-kinases (By
 CARBOHYD 245 245 N-linked (GlcNAc...).
 CARBOHYD 270 270 N-linked (GlcNAc...).
 CARBOHYD 292 292 N-linked (GlcNAc...).
 CARBOHYD 319 319 N-linked (GlcNAc...) (Potential).
 DISULFID 55 121
 DISULFID 170 228 Potential.
 DISULFID 273 331 Potential.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Disulfide bond; Glycoprotein; Immunoglobulin domain; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Repeat; SH3-binding; Signal; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 504 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0007155; P:cell adhesion; TAS:ProtInc.
 GO:0050900; P:leukocyte migration; TAS:Reactome. 

Interpro

 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold.
 IPR003597; Ig_C1-set.
 IPR003599; Ig_sub.
 IPR013106; Ig_V-set. 

Pfam

 PF07654; C1-set
 PF07686; V-set 

SMART

 SM00409; IG
 SM00407; IGc1 

PROSITE

 PS50835; IG_LIKE 

PRINTS