CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011197
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 1,4-alpha-glucan-branching enzyme 
Protein Synonyms/Alias
 Brancher enzyme; Glycogen-branching enzyme 
Gene Name
 GBE1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
39LEIDPYLKPYAVDFQubiquitination[1, 2, 3, 4]
50VDFQRRYKQFSQILKubiquitination[2]
68ENEGGIDKFSRGYESacetylation[5]
68ENEGGIDKFSRGYESubiquitination[2, 6]
143VPHGSKLKVVITSKSubiquitination[2]
149LKVVITSKSGEILYRubiquitination[1, 2, 3]
162YRISPWAKYVVREGDubiquitination[2, 6]
212GISSHEGKVASYKHFubiquitination[1, 2, 6]
217EGKVASYKHFTCNVLubiquitination[2]
442LAMAIPDKWIQLLKEubiquitination[2, 6]
472TNRRYLEKCIAYAESubiquitination[2]
554EWLDFPRKGNNESYHubiquitination[1, 2]
578DDDLLRYKFLNNFDRubiquitination[1, 2, 6]
645VGTALPGKFKIVLDSubiquitination[2]
647TALPGKFKIVLDSDAubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Required for sufficient glycogen accumulation. The alpha 1-6 branches of glycogen play an important role in increasing the solubility of the molecule and, consequently, in reducing the osmotic pressure within cells. 
Sequence Annotation
 ACT_SITE 357 357 Nucleophile (By similarity).
 ACT_SITE 412 412 Proton donor (By similarity).
 MOD_RES 173 173 Phosphotyrosine.  
Keyword
 Complete proteome; Disease mutation; Glycogen biosynthesis; Glycogen storage disease; Glycosyltransferase; Phosphoprotein; Polymorphism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 702 AA 
Protein Sequence
MAAPMTPAAR PEDYEAALNA ALADVPELAR LLEIDPYLKP YAVDFQRRYK QFSQILKNIG 60
ENEGGIDKFS RGYESFGVHR CADGGLYCKE WAPGAEGVFL TGDFNGWNPF SYPYKKLDYG 120
KWELYIPPKQ NKSVLVPHGS KLKVVITSKS GEILYRISPW AKYVVREGDN VNYDWIHWDP 180
EHSYEFKHSR PKKPRSLRIY ESHVGISSHE GKVASYKHFT CNVLPRIKGL GYNCIQLMAI 240
MEHAYYASFG YQITSFFAAS SRYGTPEELQ ELVDTAHSMG IIVLLDVVHS HASKNSADGL 300
NMFDGTDSCY FHSGPRGTHD LWDSRLFAYS SWEILRFLLS NIRWWLEEYR FDGFRFDGVT 360
SMLYHHHGVG QGFSGDYSEY FGLQVDEDAL TYLMLANHLV HTLCPDSITI AEDVSGMPAL 420
CSPISQGGGG FDYRLAMAIP DKWIQLLKEF KDEDWNMGDI VYTLTNRRYL EKCIAYAESH 480
DQALVGDKSL AFWLMDAEMY TNMSVLTPFT PVIDRGIQLH KMIRLITHGL GGEGYLNFMG 540
NEFGHPEWLD FPRKGNNESY HYARRQFHLT DDDLLRYKFL NNFDRDMNRL EERYGWLAAP 600
QAYVSEKHEG NKIIAFERAG LLFIFNFHPS KSYTDYRVGT ALPGKFKIVL DSDAAEYGGH 660
QRLDHSTDFF SEAFEHNGRP YSLLVYIPSR VALILQNVDL PN 702 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0003844; F:1,4-alpha-glucan branching enzyme activity; TAS:ProtInc.
 GO:0043169; F:cation binding; IEA:InterPro.
 GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
 GO:0006006; P:glucose metabolic process; TAS:Reactome.
 GO:0005978; P:glycogen biosynthetic process; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR006407; 1-4-A-glucan_branch_enz.
 IPR006048; A-amylase_b_C.
 IPR015902; Glyco_hydro_13.
 IPR013780; Glyco_hydro_13_b.
 IPR006047; Glyco_hydro_13_cat_dom.
 IPR004193; Glyco_hydro_13_N.
 IPR013781; Glyco_hydro_catalytic_dom.
 IPR017853; Glycoside_hydrolase_SF.
 IPR013783; Ig-like_fold.
 IPR014756; Ig_E-set. 
Pfam
 PF00128; Alpha-amylase
 PF02806; Alpha-amylase_C
 PF02922; CBM_48 
SMART
  
PROSITE
  
PRINTS