CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022319
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like 
Protein Synonyms/Alias
 Up-regulated in lung cancer protein 8; URLC8; tRNA-dihydrouridine synthase 2-like; hDUS2 
Gene Name
 DUS2L 
Gene Synonyms/Alias
 DUS2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
118DVNMGCPKQYSTKGGubiquitination[1]
123CPKQYSTKGGMGAALubiquitination[1]
146KILSTLVKGTRRPVTubiquitination[1]
155TRRPVTCKIRILPSLubiquitination[1]
170EDTLSLVKRIERTGIubiquitination[1, 2, 3, 4]
266PLEEVMQKYIRYAVQubiquitination[1]
337QQARLSAKTSEQTGEubiquitination[1, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Dihydrouridine synthase. Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Negatively regulates the activation of EIF2AK2/PKR. 
Sequence Annotation
 DOMAIN 369 436 DRBM.
 MOD_RES 488 488 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; Endoplasmic reticulum; FAD; Flavoprotein; Oxidoreductase; Phosphoprotein; Reference proteome; RNA-binding; tRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 493 AA 
Protein Sequence
MILNSLSLCY HNKLILAPMV RVGTLPMRLL ALDYGADIVY CEELIDLKMI QCKRVVNEVL 60
STVDFVAPDD RVVFRTCERE QNRVVFQMGT SDAERALAVA RLVENDVAGI DVNMGCPKQY 120
STKGGMGAAL LSDPDKIEKI LSTLVKGTRR PVTCKIRILP SLEDTLSLVK RIERTGIAAI 180
AVHGRKREER PQHPVSCEVI KAIADTLSIP VIANGGSHDH IQQYSDIEDF RQATAASSVM 240
VARAAMWNPS IFLKEGLRPL EEVMQKYIRY AVQYDNHYTN TKYCLCQMLR EQLESPQGRL 300
LHAAQSSREI CEAFGLGAFY EETTQELDAQ QARLSAKTSE QTGEPAEDTS GVIKMAVKFD 360
RRAYPAQITP KMCLLEWCRR EKLAQPVYET VQRPLDRLFS SIVTVAEQKY QSTLWDKSKK 420
LAEQAAAIVC LRSQGLPEGR LGEESPSLHK RKREAPDQDP GGPRAQELAQ PGDLCKKPFV 480
ALGSGEESPL EGW 493 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0003725; F:double-stranded RNA binding; IDA:UniProtKB.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0004860; F:protein kinase inhibitor activity; IDA:UniProtKB.
 GO:0017150; F:tRNA dihydrouridine synthase activity; IEA:InterPro.
 GO:0060548; P:negative regulation of cell death; IDA:UniProtKB.
 GO:0002943; P:tRNA dihydrouridine synthesis; IEA:GOC. 
Interpro
 IPR013785; Aldolase_TIM.
 IPR001159; Ds-RNA-bd.
 IPR014720; dsRNA-bd-like_dom.
 IPR001269; tRNA_hU_synthase.
 IPR018517; tRNA_hU_synthase_CS. 
Pfam
 PF00035; dsrm
 PF01207; Dus 
SMART
 SM00358; DSRM 
PROSITE
 PS50137; DS_RBD
 PS01136; UPF0034 
PRINTS