UniProt Accession

 PIMT_ECOLI; P0A7A5; P24206; Q2MA86 

Genbank Protein ID

 M63493; U29579; U00096; AP009048; L07942; L07869; D17549 

Genbank Nucleotide ID

 AAA24302.1; AAA69253.1; AAC75785.1; BAE76820.1; AAA79840.1; AAA17874.1; BAA04486.1 

Protein Name

 Protein-L-isoaspartate O-methyltransferase 

Protein Synonyms/Alias

 L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT 

Gene Name

 pcm 

Gene Synonyms/Alias

 b2743; JW2713 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
34	LAAVPREKFVDEAFE	acetylation	[1]
118	WQARRRLKNLDLHNV	acetylation	[1]
204	VRFVPLVKGELA***	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. This enzyme does not act on D-aspartyl residues. 

Sequence Annotation

 ACT_SITE 59 59 By similarity.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 208 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:EcoCyc.
 GO:0004719; F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity; IDA:EcoCyc.
 GO:0030091; P:protein repair; IEA:HAMAP. 

Interpro

 IPR000682; PCMT. 

Pfam

 PF01135; PCMT 

SMART

  

PROSITE

 PS01279; PCMT 

PRINTS