UniProt Accession

 GCS1_YEAST; P35197; D6VRC9 

Genbank Protein ID

 L24125; Z74274; BK006938 

Genbank Nucleotide ID

 AAA50389.1; CAA98805.1; DAA11639.1 

Protein Name

 ADP-ribosylation factor GTPase-activating protein GCS1 

Protein Synonyms/Alias

 ARF GAP GCS1 

Gene Name

 GCS1 

Gene Synonyms/Alias

 YDL226C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
19	RRLLQLQKIGANKKC	acetylation	[1]
40	NPQWATPKFGAFICL	ubiquitination	[2]
72	SITMDQFKPEELLRM	acetylation	[1]
182	SNGANFQKEKNEAYF	ubiquitination	[2]
210	LPPSQGGKYQGFGST	ubiquitination	[2]
220	GFGSTPAKPPQERSA	ubiquitination	[2]
286	GELSEETKRAAAQFG	ubiquitination	[2]
334	TTHTEYQKIDNNDKK	ubiquitination	[2]
348	KNEQDEDKWDDF***	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 GTPase-activating protein (GAP) for ARF1 and ARF2. Involved in intracellular vesicular transport. Required for transport from the trans-Golgi network. Implicated in the regulation of retrograde transport from the Golgi to the ER and in actin cytoskeletal organization. May be involved in the maintenance of mitochondrial morphology, possibly through organizing the actin cytoskeleton in Saccharomyces. 

Sequence Annotation

 DOMAIN 11 127 Arf-GAP.
 ZN_FING 26 49 C4-type.
 MOD_RES 151 151 Phosphothreonine.
 MOD_RES 157 157 Phosphoserine.
 MOD_RES 161 161 Phosphothreonine.
 MOD_RES 168 168 Phosphoserine.
 MOD_RES 170 170 Phosphothreonine.
 MOD_RES 260 260 Phosphoserine.  

Keyword

 Complete proteome; Cytoplasm; Golgi apparatus; GTPase activation; Metal-binding; Mitochondrion; Phosphoprotein; Protein transport; Reference proteome; Transport; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 352 AA 

Protein Sequence

Gene Ontology

 GO:0005856; C:cytoskeleton; IPI:SGD.
 GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IPI:SGD.
 GO:0005768; C:endosome; IDA:SGD.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005802; C:trans-Golgi network; IDA:SGD.
 GO:0003779; F:actin binding; IDA:SGD.
 GO:0008060; F:ARF GTPase activator activity; IDA:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0030037; P:actin filament reorganization involved in cell cycle; IMP:SGD.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; IGI:SGD.
 GO:0043001; P:Golgi to plasma membrane protein transport; IMP:SGD.
 GO:0032312; P:regulation of ARF GTPase activity; IEA:InterPro.
 GO:0006890; P:retrograde vesicle-mediated transport, Golgi to ER; IMP:SGD. 

Interpro

 IPR001164; ArfGAP. 

Pfam

 PF01412; ArfGap 

SMART

 SM00105; ArfGap 

PROSITE

 PS50115; ARFGAP 

PRINTS

 PR00405; REVINTRACTNG.