CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022681
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Spastin 
Protein Synonyms/Alias
  
Gene Name
 Spast 
Gene Synonyms/Alias
 Kiaa1083; Spg4 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
552SDLTALAKDAALGPIubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis (By similarity). Required for development of axonal processes and for axonal branching. 
Sequence Annotation
 DOMAIN 118 193 MIT.
 NP_BIND 380 387 ATP (Potential).
 REGION 1 298 Required for interaction with RTN1 (By
 REGION 1 192 Required for midbody localization (By
 REGION 1 78 Required for interaction with ATL1 (By
 REGION 1 48 Required for nuclear localization (By
 REGION 48 85 Required for interaction with SSNA1 and
 REGION 110 194 Sufficient for interaction with CHMP1B
 REGION 112 198 Required for interaction with
 REGION 224 326 Sufficient for interaction with
 REGION 226 614 Sufficient for microtubule severing (By
 REGION 268 326 Required for interaction with
 MOTIF 4 11 Nuclear localization signal (By
 MOTIF 57 65 Nuclear export signal (By similarity).
 MOTIF 307 310 Nuclear localization signal (By
 MOD_RES 243 243 Phosphoserine (By similarity).
 MOD_RES 266 266 Phosphoserine (By similarity).
 MOD_RES 304 304 Phosphothreonine (By similarity).  
Keyword
 ATP-binding; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Developmental protein; Differentiation; Endoplasmic reticulum; Endosome; Hydrolase; Membrane; Microtubule; Neurogenesis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 614 AA 
Protein Sequence
MSSPAGRRKK KGSGGASPAP ARPPPPAAVP APAAGPAPAA GSPPKRNPSS FSSPLVVGFA 60
LLRLLACHLG LLFAWLCQRF SRALMAAKRS SGTAPAPASP SPPEPGPGGE AESVRVFHKQ 120
AFEYISIALR IDEEEKAGQK EQAVEWYKKG IEELEKGIAV IVTGQGEQYE RARRLQAKMM 180
TNLVMAKDRL QLLEKLQPVL QFSKSQTDVY NESTNLTCRN GHLQSESGAV PKRKDPLTHA 240
SNSLPRSKTV LKSGSAGLSG HHRAPSCSGL SMVSGARPGP GPAATTHKGT PKPNRTNKPS 300
TPTTAVRKKK DLKNFRNVDS NLANLIMNEI VDNGTAVKFD DIAGQELAKQ ALQEIVILPS 360
LRPELFTGLR APARGLLLFG PPGNGKTMLA KAVAAESNAT FFNISAASLT SKYVGEGEKL 420
VRALFAVARE LQPSIIFIDE VDSLLCERRE GEHDASRRLK TEFLIEFDGV QSAGDDRVLV 480
MGATNRPQEL DEAVLRRFIK RVYVSLPNEE TRLLLLKNLL CKQGSPLTQK ELAQLARMTD 540
GYSGSDLTAL AKDAALGPIR ELKPEQVKNM SASEMRNIRL SDFTESLKKI KRSVSPQTLE 600
AYIRWNKDFG DTTV 614 
Gene Ontology
 GO:0005813; C:centrosome; IEA:HAMAP.
 GO:0031410; C:cytoplasmic vesicle; IEA:Compara.
 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005768; C:endosome; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005874; C:microtubule; IDA:MGI.
 GO:0030496; C:midbody; IEA:HAMAP.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0043014; F:alpha-tubulin binding; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0048487; F:beta-tubulin binding; ISS:UniProtKB.
 GO:0008017; F:microtubule binding; ISS:UniProtKB.
 GO:0008568; F:microtubule-severing ATPase activity; ISS:UniProtKB.
 GO:0007409; P:axonogenesis; IEA:HAMAP.
 GO:0007109; P:cytokinesis, completion of separation; ISS:UniProtKB.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; ISS:UniProtKB.
 GO:0001578; P:microtubule bundle formation; ISS:UniProtKB.
 GO:0051013; P:microtubule severing; ISS:UniProtKB.
 GO:0031117; P:positive regulation of microtubule depolymerization; IEA:HAMAP.
 GO:0034214; P:protein hexamerization; ISS:UniProtKB.
 GO:0051260; P:protein homooligomerization; ISS:UniProtKB. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR003960; ATPase_AAA_CS.
 IPR007330; MIT.
 IPR027417; P-loop_NTPase.
 IPR017179; Spastin. 
Pfam
 PF00004; AAA
 PF04212; MIT 
SMART
 SM00382; AAA
 SM00745; MIT 
PROSITE
 PS00674; AAA 
PRINTS