CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017127
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ras and EF-hand domain-containing protein 
Protein Synonyms/Alias
 Ras-related protein Rab-45 
Gene Name
 RASEF 
Gene Synonyms/Alias
 RAB45 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
171QPYEHVIKNFIREIRubiquitination[1, 2]
366ALENSYSKFNRSLHIubiquitination[1, 2]
388TISRSSPKFIGHSPQubiquitination[1, 2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Binds predominantly GDP, and also GTP. 
Sequence Annotation
 DOMAIN 8 42 EF-hand 1.
 DOMAIN 42 77 EF-hand 2.
 NP_BIND 548 555 GTP (Probable).
 NP_BIND 596 600 GTP (Probable).
 NP_BIND 654 657 GTP (By similarity).  
Keyword
 3D-structure; Alternative splicing; Calcium; Coiled coil; Complete proteome; Cytoplasm; GTP-binding; Metal-binding; Nucleotide-binding; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 740 AA 
Protein Sequence
MEADGDGEEL ARLRSVFAAC DANRSGRLER EEFRALCTEL RVRPADAEAV FQRLDADRDG 60
AITFQEFARG FLGSLRGGRR RDWGPLDPAP AVSEAGPETH DSEEDEGDED AAAALATSCG 120
PASPGRAWQD FQARLGDEAK FIPREEQVST LYQNINLVEP RLIQPYEHVI KNFIREIRLQ 180
STEMENLAIA VKRAQDKAAM QLSELEEEMD QRIQAAEHKT RKDEKRKAEE ALSDLRRQYE 240
TEVGDLQVTI KKLRKLEEQS KRVSQKEDVA ALKKQIYDLS MENQKVKKDL LEAQTNIAFL 300
QSELDALKSD YADQSLNTER DLEIIRAYTE DRNSLERQIE ILQTANRKLH DSNDGLRSAL 360
ENSYSKFNRS LHINNISPGN TISRSSPKFI GHSPQPLGYD RSSRSSYVDE DCDSLALCDP 420
LQRTNCEVDS LPESCFDSGL STLRDPNEYD SEVEYKHQRG FQRSHGVQES FGGDASDTDV 480
PDIRDEETFG LEDVASVLDW KPQGSVSEGS IVSSSRKPIS ALSPQTDLVD DNAKSFSSQK 540
AYKIVLAGDA AVGKSSFLMR LCKNEFRENI SATLGVDFQM KTLIVDGERT VLQLWDTAGQ 600
ERFRSIAKSY FRKADGVLLL YDVTCEKSFL NIREWVDMIE DAAHETVPIM LVGNKADIRD 660
TAATEGQKCV PGHFGEKLAM TYGALFCETS AKDGSNIVEA VLHLAREVKK RTDKDDSRSI 720
TNLTGTNSKK SPQMKNCCNG 740 
Gene Ontology
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0015031; P:protein transport; IEA:InterPro.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR003579; Small_GTPase_Rab_type. 
Pfam
 PF13499; EF_hand_5
 PF00071; Ras 
SMART
 SM00054; EFh
 SM00175; RAB 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS51419; RAB 
PRINTS
 PR00449; RASTRNSFRMNG.