UniProt Accession

 DOM34_YEAST; P33309; D6W1H6 

Genbank Protein ID

 L11277; X77114; Z71277; BK006947 

Genbank Nucleotide ID

 AAA34575.1; CAA54375.1; CAA95860.1; DAA10542.1 

Protein Name

 Protein DOM34 

Protein Synonyms/Alias

  

Gene Name

 DOM34 

Gene Synonyms/Alias

 YNL001W; N2016 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
183	KRTTDVLKFDEKTEK	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Involved in protein translation. Together with HBS1, may function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non- functional ribosomes and degrade damaged mRNAs. The complex formed by DOM34 and HBS1 has ribonuclease activity towards double- stranded RNA substrates, but does not cleave single-stranded RNA. Acts as endonuclease; has no exonuclease activity. Increases the affinity of HBS1 for GTP, but nor for GDP. Promotes G1 progression and differentiation and is involved in mitotic and meiotic cell divisions. 

Sequence Annotation

  

Keyword

 3D-structure; Cell cycle; Cell division; Complete proteome; Cytoplasm; Endonuclease; Hydrolase; Meiosis; Metal-binding; Mitosis; Nuclease; Protein biosynthesis; Reference proteome; Translation regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 386 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0004521; F:endoribonuclease activity; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007126; P:meiosis; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0070651; P:nonfunctional rRNA decay; IMP:SGD.
 GO:0000294; P:nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay; IDA:SGD.
 GO:0070966; P:nuclear-transcribed mRNA catabolic process, no-go decay; IMP:SGD.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0032790; P:ribosome disassembly; IDA:SGD.
 GO:0006412; P:translation; IEA:UniProtKB-KW. 

Interpro

 IPR005140; eRF1_1_Pelota.
 IPR005141; eRF1_2.
 IPR005142; eRF1_3.
 IPR004405; Transl_rel_pelota-like. 

Pfam

 PF03463; eRF1_1
 PF03464; eRF1_2
 PF03465; eRF1_3 

SMART

  

PROSITE

  

PRINTS