CPLM-000908

Genbank Nucleotide ID

Leucine carboxyl methyltransferase 2

Protein Synonyms/Alias

p21WAF1/CIP1 promoter-interacting protein; tRNA wybutosine-synthesizing protein 4 homolog; tRNA yW-synthesizing protein 4 homolog

Homo sapiens (Human)

Position	Peptide	Type	References
24	NDSSALSKRSLAARG	ubiquitination	[1, 2, 3, 4]
356	EGQVPNLKRYGHASV	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
401	RDCDSEWKGSQIGSC	ubiquitination	[3, 5]
463	DNNTEDLKVTITKAG	ubiquitination	[1, 3]
468	DLKVTITKAGRKDDS	ubiquitination	[1, 3]
472	TITKAGRKDDSTLCC	ubiquitination	[3]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[5] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA (By similarity). May methylate the carboxyl group of leucine residues to form alpha- leucine ester residues.

REGION 161 162 S-adenosyl-L-methionine binding (By
BINDING 59 59 S-adenosyl-L-methionine (By similarity).
BINDING 89 89 S-adenosyl-L-methionine; via carbonyl
BINDING 114 114 S-adenosyl-L-methionine (By similarity).
BINDING 188 188 S-adenosyl-L-methionine; via carbonyl

Complete proteome; Methyltransferase; Polymorphism; Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.

IPR015915; Kelch-typ_b-propeller.
IPR007213; LCM_MeTrfase.
IPR021121; LCM_MeTrfase_euk.