UniProt Accession

 NB5R3_HUMAN; P00387; B1AHF2; B7Z7L3; O75675; Q8TDL8; Q8WTS8; Q9UEN4; Q9UEN5; Q9UL55; Q9UL56 

Genbank Protein ID

 M28713; M28705; M28706; M28707; M28708; M28709; M28710; M28711; Y09501; AF361370; AJ010116; AJ010117; AJ010118; AY341030; BT009821; CR456435; AF061830; AF061831; AK302204; Z93241; Z93241; BC004821; AJ310899; AJ310900; M16461; M16462 

Genbank Nucleotide ID

 AAA59900.1; AAA59900.1; AAA59900.1; AAA59900.1; AAA59900.1; AAA59900.1; AAA59900.1; AAA59900.1; CAA70696.1; AAL87744.1; CAA09006.1; CAA09007.1; CAA09008.1; AAP88936.1; AAP88823.1; CAG30321.1; AAF06818.1; AAF06819.1; BAH13649.1; CAB42843.1; CAQ08414.1; AAH04821.1; CAC84523.1; CAC84524.1; AAA52306.1; AAA52307.1 

Protein Name

 NADH-cytochrome b5 reductase 3 

Protein Synonyms/Alias

 B5R; Cytochrome b5 reductase; Diaphorase-1; NADH-cytochrome b5 reductase 3 membrane-bound form; NADH-cytochrome b5 reductase 3 soluble form 

Gene Name

 CYB5R3 

Gene Synonyms/Alias

 DIA1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
19	TLESPDIKYPLRLID	ubiquitination	[1]
92	LVIKVYFKDTHPKFP	ubiquitination	[1]
97	YFKDTHPKFPAGGKM	ubiquitination	[1]
103	PKFPAGGKMSQYLES	ubiquitination	[1]
140	KFAIRPDKKSNPIIR	ubiquitination	[1]
150	NPIIRTVKSVGMIAG	ubiquitination	[1]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. 

Sequence Annotation

 DOMAIN 40 152 FAD-binding FR-type.
 NP_BIND 132 147 FAD (By similarity).
 NP_BIND 171 206 FAD (By similarity).
 MOD_RES 42 42 N6-acetyllysine.
 MOD_RES 43 43 Phosphotyrosine.
 MOD_RES 120 120 N6-acetyllysine (By similarity).
 MOD_RES 130 130 Phosphotyrosine (By similarity).
 LIPID 2 2 N-myristoyl glycine.  

Keyword

 3D-structure; Acetylation; Alternative promoter usage; Alternative splicing; Cholesterol biosynthesis; Cholesterol metabolism; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; Endoplasmic reticulum; FAD; Flavoprotein; Lipid biosynthesis; Lipid metabolism; Lipoprotein; Membrane; Mitochondrion; Mitochondrion outer membrane; Myristate; NAD; Oxidoreductase; Phosphoprotein; Polymorphism; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 301 AA 

Protein Sequence

Gene Ontology

 GO:0005783; C:endoplasmic reticulum; IDA:HPA.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0005833; C:hemoglobin complex; TAS:ProtInc.
 GO:0005811; C:lipid particle; IDA:UniProtKB.
 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0005741; C:mitochondrial outer membrane; TAS:Reactome.
 GO:0043531; F:ADP binding; IEA:Compara.
 GO:0016208; F:AMP binding; IEA:Compara.
 GO:0004128; F:cytochrome-b5 reductase activity, acting on NAD(P)H; TAS:Reactome.
 GO:0071949; F:FAD binding; IDA:UniProtKB.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:Compara.
 GO:0051287; F:NAD binding; IEA:Compara.
 GO:0008015; P:blood circulation; TAS:ProtInc.
 GO:0006695; P:cholesterol biosynthetic process; IEA:UniProtKB-KW.
 GO:0019852; P:L-ascorbic acid metabolic process; TAS:Reactome. 

Interpro

 IPR017927; Fd_Rdtase_FAD-bd.
 IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
 IPR001834; NADH-Cyt_B5_reductase.
 IPR008333; OxRdtase_FAD-bd_dom.
 IPR001433; OxRdtase_FAD/NAD-bd.
 IPR017938; Riboflavin_synthase-like_b-brl. 

Pfam

 PF00970; FAD_binding_6
 PF00175; NAD_binding_1 

SMART

  

PROSITE

 PS51384; FAD_FR 

PRINTS

 PR00406; CYTB5RDTASE.
 PR00371; FPNCR.