UniProt Accession

 ORC4_HUMAN; O43929; B7Z3D0; B7Z5F1; D3DP86; F5H069; Q96C42 

Genbank Protein ID

 AF022108; AF047598; AF132596; AY600302; AK295721; AK298862; AC009480; AC019226; CH471058; CH471058; CH471058; CH471058; BC014847 

Genbank Nucleotide ID

 AAC01957.1; AAC80282.1; AAD22110.1; AAS94326.1; BAH12166.1; BAH12887.1; EAX11555.1; EAX11556.1; EAX11557.1; EAX11558.1; AAH14847.1 

Protein Name

 Origin recognition complex subunit 4 

Protein Synonyms/Alias

  

Gene Name

 ORC4 

Gene Synonyms/Alias

 ORC4L 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
112	INDKIALKEITRQLN	ubiquitination	[1]
204	DILELLEKRVKSRFS	ubiquitination	[1, 2, 3, 4, 5]
231	PQYVKIFKEQLSLPA	ubiquitination	[1]
243	LPAEFPDKVFAEKWN	ubiquitination	[2, 5]
248	PDKVFAEKWNENVQY	ubiquitination	[1, 6]
274	QKHFNISKNLRSLHM	ubiquitination	[1]
386	LQQLELIKPMERTSG	ubiquitination	[1, 6]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. 

Sequence Annotation

 NP_BIND 67 74 ATP (Potential).  

Keyword

 Alternative splicing; ATP-binding; Complete proteome; Disease mutation; DNA replication; DNA-binding; Dwarfism; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 436 AA 

Protein Sequence

Gene Ontology

 GO:0005664; C:nuclear origin of replication recognition complex; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003688; F:DNA replication origin binding; IMP:UniProtKB.
 GO:0017111; F:nucleoside-triphosphatase activity; IEA:InterPro.
 GO:0000166; F:nucleotide binding; IDA:UniProtKB.
 GO:0006270; P:DNA replication initiation; IMP:UniProtKB.
 GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:Reactome. 

Interpro

 IPR003593; AAA+_ATPase.
 IPR016527; ORC4.
 IPR027417; P-loop_NTPase. 

Pfam

  

SMART

 SM00382; AAA 

PROSITE

  

PRINTS