UniProt Accession

 GLMU_MYCTU; P96382; L0T872; Q7D8Z8 

Genbank Protein ID

 AE000516; AL123456 

Genbank Nucleotide ID

 AAK45297.1; CCP43768.1 

Protein Name

 Bifunctional protein GlmU 

Protein Synonyms/Alias

 UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase 

Gene Name

 glmU 

Gene Synonyms/Alias

 Rv1018c; MT1046 

Created Date

 July 27, 2013 

Organism

 Mycobacterium tuberculosis 

NCBI Taxa ID

 1773 

Lysine Modification

Position	Peptide	Type	References
362	LGAFVEVKNSTIGTG	pupylation	[1]

Reference

 [1] Prokaryotic ubiquitin-like protein (Pup) proteome of Mycobacterium tuberculosis [corrected] .
 Festa RA, McAllister F, Pearce MJ, Mintseris J, Burns KE, Gygi SP, Darwin KH.
 PLoS One. 2010 Jan 6;5(1):e8589. [PMID: 20066036] 

Functional Description

 Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP- GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5- monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain. 

Sequence Annotation

 REGION 1 241 Pyrophosphorylase (By similarity).
 REGION 12 15 UDP-GlcNAc binding.
 REGION 88 89 UDP-GlcNAc binding.
 REGION 112 114 UDP-GlcNAc binding.
 REGION 242 262 Linker (By similarity).
 REGION 263 495 N-acetyltransferase (By similarity).
 REGION 397 398 Acetyl-CoA binding (By similarity).
 ACT_SITE 374 374 Proton acceptor (By similarity).
 METAL 114 114 Magnesium.
 METAL 239 239 Magnesium.
 BINDING 26 26 UDP-GlcNAc.
 BINDING 83 83 UDP-GlcNAc.
 BINDING 151 151 UDP-GlcNAc; via amide nitrogen.
 BINDING 166 166 UDP-GlcNAc.
 BINDING 181 181 UDP-GlcNAc.
 BINDING 239 239 UDP-GlcNAc.
 BINDING 344 344 UDP-GlcNAc (By similarity).
 BINDING 362 362 UDP-GlcNAc (By similarity).
 BINDING 377 377 UDP-GlcNAc (By similarity).
 BINDING 388 388 UDP-GlcNAc (By similarity).
 BINDING 391 391 Acetyl-CoA; via amide nitrogen.
 BINDING 416 416 Acetyl-CoA; via carbonyl oxygen.
 BINDING 434 434 Acetyl-CoA; via amide nitrogen.
 CROSSLNK 362 362 Isoglutamyl lysine isopeptide (Lys-Gln)  

Keyword

 3D-structure; Acyltransferase; Cell shape; Cell wall biogenesis/degradation; Complete proteome; Cytoplasm; Isopeptide bond; Magnesium; Metal-binding; Multifunctional enzyme; Nucleotidyltransferase; Peptidoglycan synthesis; Reference proteome; Repeat; Transferase; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 495 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0019134; F:glucosamine-1-phosphate N-acetyltransferase activity; IDA:MTBBASE.
 GO:0000287; F:magnesium ion binding; IDA:MTBBASE.
 GO:0003977; F:UDP-N-acetylglucosamine diphosphorylase activity; IDA:MTBBASE.
 GO:0000902; P:cell morphogenesis; IEA:HAMAP.
 GO:0040007; P:growth; IMP:MTBBASE.
 GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:InterPro.
 GO:0009252; P:peptidoglycan biosynthetic process; IEA:HAMAP.
 GO:0070207; P:protein homotrimerization; IPI:MTBBASE.
 GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
 GO:0006048; P:UDP-N-acetylglucosamine biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR005882; Bifunctional_GlmU.
 IPR001451; Hexapep_transf.
 IPR025877; MobA-like_NTP_Trfase_dom.
 IPR011004; Trimer_LpxA-like. 

Pfam

 PF00132; Hexapep
 PF12804; NTP_transf_3 

SMART

  

PROSITE

 PS00101; HEXAPEP_TRANSFERASES 

PRINTS