UniProt Accession

 RCSB_ECOLI; P69407; P14374 

Genbank Protein ID

 M28242; L11272; U00096; AP009048; S37760 

Genbank Nucleotide ID

 AAA24504.1; AAA24506.1; AAC75277.1; BAA16000.1; AAB22290.1 

Protein Name

 Capsular synthesis regulator component B 

Protein Synonyms/Alias

  

Gene Name

 rcsB 

Gene Synonyms/Alias

 b2217; JW2205 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
63	DLSMPGDKYGDGITL	acetylation	[1]
72	GDGITLIKYIKRHFP	acetylation	[1]
118	GAPTDLPKALAALQK	acetylation	[1]
125	KALAALQKGKKFTPE	acetylation	[1]
140	SVSRLLEKISAGGYG	acetylation	[1]
149	SAGGYGDKRLSPKES	acetylation	[1]
154	GDKRLSPKESEVLRL	acetylation	[1]
173	FLVTEIAKKLNRSIK	acetylation	[1]
180	KKLNRSIKTISSQKK	acetylation	[2]
215	VTLSPADKD******	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Nε-lysine acetylation of a bacterial transcription factor inhibits Its DNA-binding activity.
 Thao S, Chen CS, Zhu H, Escalante-Semerena JC.
 PLoS One. 2010 Dec 31;5(12):e15123. [PMID: 21217812] 

Functional Description

 Member of the two-component regulatory system RcsB/RcsC, which regulates the expression of genes involved in colanic acid capsule synthesis. The two-component system interacts with a phosphotransfer intermediate, RcsD, which receives a phosphoryl group from RcsC and transfers it to RscB. RcsB acts as the effector. Additional control is provided by the dependence on the alternate sigma factor, RpoN, for the synthesis of RcsB. Activity is probably up-regulated by YmgA/AriR, and possibly down-regulated by YcgZ, all 3 are connector proteins providing additional signal input into the two-component system. 

Sequence Annotation

 DOMAIN 1 124 Response regulatory.
 DOMAIN 144 209 HTH luxR-type.
 DNA_BIND 168 187 H-T-H motif (By similarity).
 MOD_RES 56 56 4-aspartylphosphate (By similarity).  

Keyword

 Activator; Capsule biogenesis/degradation; Complete proteome; DNA-binding; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Two-component regulatory system. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 216 AA 

Protein Sequence

Gene Ontology

 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0000156; F:phosphorelay response regulator activity; IEA:InterPro.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0035556; P:intracellular signal transduction; IEA:GOC.
 GO:0006351; P:transcription, DNA-dependent; IDA:EcoCyc. 

Interpro

 IPR011006; CheY-like_superfamily.
 IPR016032; Sig_transdc_resp-reg_C-effctor.
 IPR001789; Sig_transdc_resp-reg_receiver.
 IPR000792; Tscrpt_reg_LuxR_C.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF00196; GerE
 PF00072; Response_reg 

SMART

 SM00421; HTH_LUXR
 SM00448; REC 

PROSITE

 PS00622; HTH_LUXR_1
 PS50043; HTH_LUXR_2
 PS50110; RESPONSE_REGULATORY 

PRINTS

 PR00038; HTHLUXR.