CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020148
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Trichoplein keratin filament-binding protein 
Protein Synonyms/Alias
 Protein TCHP; Mitochondrial protein with oncostatic activity; Mitostatin; Tumor suppressor protein 
Gene Name
 TCHP 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
50MSDICSSKQAEWSSKubiquitination[1]
57KQAEWSSKTSYQRSMubiquitination[1]
170VNSWEMQKEEKKQQEubiquitination[1]
214ERRQLEDKLQAEALLubiquitination[1]
259ERLEEERKQMEAFRQubiquitination[1]
306ILQALLEKEDESQRLubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Tumor suppressor which has the ability to inhibit cell growth and be pro-apoptotic during cell stress. Inhibits cell growth in bladder and prostate cancer cells by a down-regulation of HSPB1 by inhibiting its phosphorylation. May act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. May regulate K8/K18 filament and desmosome organization mainly at the apical or peripheral regions of simple epithelial cells. 
Sequence Annotation
 REGION 73 498 Interaction with keratin proteins.
 REGION 259 425 Trichohyalin/plectin homology domain.  
Keyword
 Apoptosis; Cell membrane; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Membrane; Mitochondrion; Polymorphism; Reference proteome; Tumor suppressor. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 498 AA 
Protein Sequence
MALPTLPSYW CSQQRLNQQL ARQREQEARL RQQWEQNSRY FRMSDICSSK QAEWSSKTSY 60
QRSMHAYQRE KMKEEKRRSL EARREKLRQL MQEEQDLLAR ELEELRLSMN LQERRIREQH 120
GKLKSAKEEQ RKLIAEQLLY EHWKKNNPKL REMELDLHQK HVVNSWEMQK EEKKQQEATA 180
EQENKRYENE YERARREALE RMKAEEERRQ LEDKLQAEAL LQQMEELKLK EVEATKLKKE 240
QENLLKQRWE LERLEEERKQ MEAFRQKAEL GRFLRHQYNA QLSRRTQQIQ EELEADRRIL 300
QALLEKEDES QRLHLARREQ VMADVAWMKQ AIEEQLQLER AREAELQMLL REEAKEMWEK 360
REAEWARERS ARDRLMSEVL TGRQQQIQEK IEQNRRAQEE SLKHREQLIR NLEEVRELAR 420
REKEESEKLK SARKQELEAQ VAERRLQAWE ADQQEEEEEE EARRVEQLSD ALLQQEAETM 480
AEQGYRPKPY GHPKIAWN 498 
Gene Ontology
 GO:0045179; C:apical cortex; IDA:HGNC.
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0045095; C:keratin filament; IDA:HGNC.
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0006915; P:apoptotic process; IDA:UniProtKB.
 GO:0030308; P:negative regulation of cell growth; IDA:UniProtKB. 
Interpro
 IPR026773; TCHP/CCDC11. 
Pfam
  
SMART
  
PROSITE
  
PRINTS