UniProt Accession

 EIF3E_MOUSE; P60229; O43902; Q64058; Q64059; Q64252 

Genbank Protein ID

 S75221; S75223; BC029177 

Genbank Nucleotide ID

 AAC00046.1; AAC00047.1; AAH29177.1 

Protein Name

 Eukaryotic translation initiation factor 3 subunit E 

Protein Synonyms/Alias

 eIF3e; Eukaryotic translation initiation factor 3 subunit 6; MMTV integration site 6; Mammary tumor-associated protein INT-6; Viral integration site protein INT-6; eIF-3 p48 

Gene Name

 Eif3e 

Gene Synonyms/Alias

 Eif3s6; Int6 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
35	VKEIYNEKELLQGKL	ubiquitination	[1]
82	TTVVAQLKQLQAETE	ubiquitination	[1]
93	AETEPIVKMFEDPET	ubiquitination	[1]
275	RKRRQVLKDLVKVIQ	acetylation	[2]
275	RKRRQVLKDLVKVIQ	ubiquitination	[1]
387	LDAKIDSKLGHVVMG	ubiquitination	[1]
407	PYQQVIEKTKSLSFR	ubiquitination	[1]
409	QQVIEKTKSLSFRSQ	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins (By similarity). Component of the eukaryotic translation initiation factor 3 (eIF- 3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF- 2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. 

Sequence Annotation

 DOMAIN 290 395 PCI.
 REGION 4 128 Sufficient for interaction with EPAS1 (By
 REGION 9 195 Sufficient for interaction with TRIM27
 REGION 351 445 Sufficient for interaction with MCM7 (By
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 275 275 N6-acetyllysine (By similarity).
 MOD_RES 399 399 Phosphoserine (By similarity).
 MOD_RES 439 439 Phosphothreonine.
 MOD_RES 445 445 Phosphotyrosine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Initiation factor; Nucleus; Phosphoprotein; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 445 AA 

Protein Sequence

Gene Ontology

 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; IDA:UniProtKB.
 GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; ISS:UniProtKB.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP.
 GO:0006413; P:translational initiation; IDA:UniProtKB. 

Interpro

 IPR016650; eIF3e.
 IPR019010; eIF3e_N.
 IPR000717; PCI_dom.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF09440; eIF3_N
 PF01399; PCI 

SMART

 SM00088; PINT 

PROSITE

  

PRINTS