CPLM-022288

Genbank Nucleotide ID

Probable tRNA(His) guanylyltransferase

Protein Synonyms/Alias

Interphase cytoplasmic foci protein 45; tRNA-histidine guanylyltransferase

Homo sapiens (Human)

Position	Peptide	Type	References
68	NFHRFAEKHNFAKPN	acetylation	[1]
119	KRKTNWFKRRASKFM	ubiquitination	[2, 3]
170	YPSNQTLKDYLSWRQ	ubiquitination	[4]
252	KVDEVMTKEIKLPTE	ubiquitination	[5]
263	LPTEMEGKKMAVTRT	acetylation	[6, 7, 8, 9]

[1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[6] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
[7] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
[8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis.

NP_BIND 58 63 GTP.
NP_BIND 104 105 GTP.
METAL 58 58 Magnesium 1; catalytic.
METAL 58 58 Magnesium 2; catalytic.
METAL 59 59 Magnesium 1; via carbonyl oxygen;
METAL 105 105 Magnesium 1; catalytic.
METAL 105 105 Magnesium 2; catalytic.
MOD_RES 251 251 Phosphothreonine (By similarity).

3D-structure; Complete proteome; Cytoplasm; GTP-binding; Magnesium; Metal-binding; Nucleotide-binding; Nucleotidyltransferase; Phosphoprotein; Polymorphism; Reference proteome; Transferase; tRNA processing.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005739; C:mitochondrion; IDA:LIFEdb.
GO:0005524; F:ATP binding; IDA:BHF-UCL.
GO:0005525; F:GTP binding; IDA:BHF-UCL.
GO:0000287; F:magnesium ion binding; IDA:BHF-UCL.
GO:0000049; F:tRNA binding; TAS:BHF-UCL.
GO:0008193; F:tRNA guanylyltransferase activity; IDA:UniProtKB.
GO:0051289; P:protein homotetramerization; IPI:UniProtKB.
GO:0006400; P:tRNA modification; ISS:UniProtKB.

IPR025845; Thg1_C_dom.
IPR024956; tRNAHis_GuaTrfase_cat.
IPR007537; tRNAHis_GuaTrfase_Thg1.

PF04446; Thg1
PF14413; Thg1C