UniProt Accession

 PDI_YEAST; P17967; D6VQX3 

Genbank Protein ID

 D00842; X57712; M62815; X52313; M76982; X54535; X59720; BK006937 

Genbank Nucleotide ID

 BAA00723.1; CAA40883.1; AAA34848.1; CAA36550.1; AAA35169.1; CAA38402.1; CAA42373.1; DAA07442.1 

Protein Name

 Protein disulfide-isomerase 

Protein Synonyms/Alias

 PDI; Thioredoxin-related glycoprotein 1 

Gene Name

 PDI1 

Gene Synonyms/Alias

 MFP1; TRG1; YCL043C; YCL313; YCL43C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
112	FPSLKIFKNSDVNNS	ubiquitination	[1]
347	DKIVLESKAIESLVK	acetylation	[2]
354	KAIESLVKDFLKGDA	acetylation	[2]
358	SLVKDFLKGDASPIV	acetylation	[2]
394	DEIVNDPKKDVLVLY	acetylation	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Participates in the folding of proteins containing disulfide bonds, may be involved in glycosylation, prolyl hydroxylation and triglyceride transfer. 

Sequence Annotation

 DOMAIN 29 141 Thioredoxin 1.
 DOMAIN 356 485 Thioredoxin 2.
 MOTIF 519 522 Prevents secretion from ER.
 ACT_SITE 61 61 Nucleophile (By similarity).
 ACT_SITE 64 64 Nucleophile (By similarity).
 ACT_SITE 406 406 Nucleophile (By similarity).
 ACT_SITE 409 409 Nucleophile (By similarity).
 CARBOHYD 82 82 N-linked (GlcNAc...) (Potential).
 CARBOHYD 117 117 N-linked (GlcNAc...) (Potential).
 CARBOHYD 155 155 N-linked (GlcNAc...) (Potential).
 CARBOHYD 174 174 N-linked (GlcNAc...) (Potential).
 CARBOHYD 425 425 N-linked (GlcNAc...) (Potential).
 DISULFID 61 64 Redox-active.
 DISULFID 406 409 Redox-active.  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; Glycoprotein; Isomerase; Redox-active center; Reference proteome; Repeat; Signal. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 522 AA 

Protein Sequence

Gene Ontology

 GO:0005788; C:endoplasmic reticulum lumen; IDA:SGD.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0003756; F:protein disulfide isomerase activity; IDA:SGD.
 GO:0015035; F:protein disulfide oxidoreductase activity; IDA:SGD.
 GO:0045454; P:cell redox homeostasis; IEA:InterPro.
 GO:0006662; P:glycerol ether metabolic process; IEA:InterPro. 

Interpro

 IPR005792; Prot_disulphide_isomerase.
 IPR005746; Thioredoxin.
 IPR012336; Thioredoxin-like_fold.
 IPR017937; Thioredoxin_CS.
 IPR013766; Thioredoxin_domain. 

Pfam

 PF00085; Thioredoxin 

SMART

  

PROSITE

 PS00014; ER_TARGET
 PS00194; THIOREDOXIN_1
 PS51352; THIOREDOXIN_2 

PRINTS

 PR00421; THIOREDOXIN.