CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030899
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Squalene synthase 
Protein Synonyms/Alias
 cDNA FLJ50548, highly similar to Squalene synthetase (EC 2.5.1.21) 
Gene Name
 FDFT1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
5***MEFVKCLGHPEEubiquitination[1, 2, 3]
30GKRKVMPKMDQDSLSubiquitination[2]
41DSLSSSLKTCYKYLNubiquitination[1, 2, 3]
45SSLKTCYKYLNQTSRubiquitination[1, 2]
92DMTISVEKKVPLLHNubiquitination[4]
93MTISVEKKVPLLHNFubiquitination[2]
115DWRFMESKEKDRQVLubiquitination[2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 374 AA 
Protein Sequence
MEFVKCLGHP EEFYNLVRFR IGGKRKVMPK MDQDSLSSSL KTCYKYLNQT SRSFAAVIQA 60
LDGEMRNAVC IFYLVLRALD TLEDDMTISV EKKVPLLHNF HSFLYQPDWR FMESKEKDRQ 120
VLEDFPTYCH YVAGLVGIGL SRLFSASEFE DPLVGEDTER ANSMGLFLQK TNIIRDYLED 180
QQGGREFWPQ EVWSRYVKKL GDFAKPENID LAVQCLNELI TNALHHIPDV ITYLSRLRNQ 240
SVFNFCAIPQ VMAIATLAAC YNNQQVFKGA VKIRKGQAVT LMMDATNMPA VKAIIYQYME 300
EIYHRIPDSD PSSSKTRQII STIRTQNLPN CQLISRSHYS PIYLSFVMLL AALSWQYLTT 360
LSQVTEDYVQ TGEH 374 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:HPA.
 GO:0016021; C:integral to membrane; IEA:InterPro.
 GO:0004310; F:farnesyl-diphosphate farnesyltransferase activity; IEA:InterPro.
 GO:0008610; P:lipid biosynthetic process; IEA:InterPro. 
Interpro
 IPR002060; Squ/phyt_synthse.
 IPR006449; Squal_synth.
 IPR019845; Squalene/phytoene_synthase_CS.
 IPR008949; Terpenoid_synth. 
Pfam
 PF00494; SQS_PSY 
SMART
  
PROSITE
 PS01044; SQUALEN_PHYTOEN_SYN_1
 PS01045; SQUALEN_PHYTOEN_SYN_2 
PRINTS