CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000380
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 1-acyl-sn-glycerol-3-phosphate acyltransferase beta 
Protein Synonyms/Alias
 1-acylglycerol-3-phosphate O-acyltransferase 2; 1-AGP acyltransferase 2; 1-AGPAT 2; Lysophosphatidic acid acyltransferase beta; LPAAT-beta 
Gene Name
 AGPAT2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
71GWFVRSFKYFYGLRFubiquitination[1]
166RMVRENLKVWIYPEGubiquitination[1, 2, 3, 4]
185NGDLLPFKKGAFYLAubiquitination[2, 3]
186GDLLPFKKGAFYLAVubiquitination[1, 2, 3]
261TTFLHISKTPQENGAubiquitination[1, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. 
Sequence Annotation
 MOTIF 98 103 HXXXXD motif.
 MOD_RES 255 255 Phosphothreonine (By similarity).
 MOD_RES 260 260 Phosphoserine (By similarity).  
Keyword
 Acyltransferase; Alternative splicing; Complete proteome; Congenital generalized lipodystrophy; Diabetes mellitus; Disease mutation; Lipid biosynthesis; Lipid metabolism; Membrane; Phospholipid biosynthesis; Phospholipid metabolism; Phosphoprotein; Reference proteome; Transferase; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 278 AA 
Protein Sequence
MELWPCLAAA LLLLLLLVQL SRAAEFYAKV ALYCALCFTV SAVASLVCLL RHGGRTVENM 60
SIIGWFVRSF KYFYGLRFEV RDPRRLQEAR PCVIVSNHQS ILDMMGLMEV LPERCVQIAK 120
RELLFLGPVG LIMYLGGVFF INRQRSSTAM TVMADLGERM VRENLKVWIY PEGTRNDNGD 180
LLPFKKGAFY LAVQAQVPIV PVVYSSFSSF YNTKKKFFTS GTVTVQVLEA IPTSGLTAAD 240
VPALVDTCHR AMRTTFLHIS KTPQENGATA GSGVQPAQ 278 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0003841; F:1-acylglycerol-3-phosphate O-acyltransferase activity; NAS:UniProtKB.
 GO:0016024; P:CDP-diacylglycerol biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006654; P:phosphatidic acid biosynthetic process; IGI:BHF-UCL.
 GO:0001819; P:positive regulation of cytokine production; IMP:BHF-UCL.
 GO:0001961; P:positive regulation of cytokine-mediated signaling pathway; IC:BHF-UCL.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0019432; P:triglyceride biosynthetic process; TAS:Reactome. 
Interpro
 IPR004552; AGP_acyltrans.
 IPR002123; Plipid/glycerol_acylTrfase. 
Pfam
 PF01553; Acyltransferase 
SMART
 SM00563; PlsC 
PROSITE
  
PRINTS