CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029761
UniProt Accession
Genbank Protein ID
 Z83840 
Genbank Nucleotide ID
  
Protein Name
 NHP2-like protein 1 
Protein Synonyms/Alias
  
Gene Name
 NHP2L1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
13TEADVNPKAYPLADAubiquitination[1, 2, 3, 4]
24LADAHLTKKLLDLVQubiquitination[1, 5]
25ADAHLTKKLLDLVQQacetylation[4]
25ADAHLTKKLLDLVQQubiquitination[2, 3, 4]
37VQQSCNYKQLRKGANmethylation[6]
37VQQSCNYKQLRKGANubiquitination[1, 2, 3, 4, 5, 7, 8, 9]
41CNYKQLRKGANEATKubiquitination[3, 4]
48KGANEATKTLNRGISubiquitination[2, 3, 4, 7, 8, 9]
111IACSVTIKEGSQLKQubiquitination[3]
117IKEGSQLKQQIQSIQubiquitination[3, 4, 7, 8, 9]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] A general molecular affinity strategy for global detection and proteomic analysis of lysine methylation.
 Moore KE, Carlson SM, Camp ND, Cheung P, James RG, Chua KF, Wolf-Yadlin A, Gozani O.
 Mol Cell. 2013 May 9;50(3):444-56. [PMID: 23583077]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 132 AA 
Protein Sequence
MLLVQTEADV NPKAYPLADA HLTKKLLDLV QQSCNYKQLR KGANEATKTL NRGISEFIVM 60
AADAEPLEII LHLPLLCEDK NVPYVFVRSK QALGRACGVS RPVIACSVTI KEGSQLKQQI 120
QSIQQSIERL LV 132 
Gene Ontology
 GO:0005730; C:nucleolus; IEA:InterPro.
 GO:0030529; C:ribonucleoprotein complex; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0042254; P:ribosome biogenesis; IEA:InterPro. 
Interpro
 IPR002415; H/ACA_rnp_Nhp2_euk.
 IPR004038; Ribosomal_L7Ae/L30e/S12e/Gad45.
 IPR018492; Ribosomal_L7Ae/L8/Nhp2.
 IPR004037; Ribosomal_L7Ae_CS. 
Pfam
 PF01248; Ribosomal_L7Ae 
SMART
  
PROSITE
 PS01082; RIBOSOMAL_L7AE 
PRINTS
 PR00881; L7ARS6FAMILY.
 PR00883; NUCLEARHMG.