CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006827
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 AP-2 complex subunit alpha 
Protein Synonyms/Alias
 Alpha-adaptin; Clathrin assembly protein complex 2 alpha large chain; Clathrin assembly protein large alpha chain 
Gene Name
 APL3 
Gene Synonyms/Alias
 YBL037W; YBL0412 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
415KLCSSSGKPAIDAVRubiquitination[1]
707STPDLLSKRANSSRSubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Alpha adaptin is a subunit of the plasma membrane adaptor. Facilitates interaction between APL1 and APS2. 
Sequence Annotation
 MOD_RES 727 727 Phosphothreonine.
 MOD_RES 733 733 Phosphoserine.  
Keyword
 Cell membrane; Coated pit; Complete proteome; Membrane; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1025 AA 
Protein Sequence
MDRKKTLINS SVSNNNSTIK GLQLFIADLR SAQQAQEQEK RIQSEIVKIK QHFDAAKKKQ 60
GNHDRLGGYQ RKKYVAKLAY IYITSNTTKL NEILFGLEQT VELLKSSIFS EKFIGYMTLE 120
LLYERSEVVA KVNDEVNYQL MKDLSSSDDN FVMLALNFVG VVGELTNRLA YNDDITTGVF 180
KILRSPTSSI YLKKKSALSF LALLKSNHSI LTEDLQRKQL WIQRILSLLD DTENYRLTLA 240
TIPLIEFIAK YIDPSYCTRL LPQLTEILYN CVVVGTSRSS DNQFPLEYTF ANMPNPWLIT 300
KVVSLLSILI ASPTERDSGS LLQTNNIDNE LLNKLRKCVS VAIELGTRQA QDPMERIVQN 360
TVLFSLINFA SKLDPSDEAI SNSVTALCSL LTSKEINIRY LTLDSLVKLC SSSGKPAIDA 420
VRYKNLDMIF HLLNTERDSS IVRKVVDLLY TFTDVENVKI IVDGLLQYIL SPKNLAEPQI 480
KSDIAVKIAI LTEKYATDIN WFVIISLQLL SLTSNTTIND DEIWQRLCQI VVNNPSLHRI 540
TCERLVDYLC KKQASEAIIK AAAFLLGEYS SLITDRISSA NLFTLFAEKY FSAPNVAKAM 600
ILTTMIKLYK TSPEIGSNVI KFFQLELNSL DIELQTRSFE YLNIIQLAKV NGNTDILQIL 660
FEPMPPFNSK SNPLLKRLGS LPASAGSTTL INTPSEASSS TPDLLSKRAN SSRSIMVPMP 720
PPSRRNTIDD VNSKISSSED FSGKDSYYSR QILAPNWREG FTRMISHKQG VLFTSSLMKV 780
FYRITTPDAQ QPYVFHISLA FINLTEWEIT GLSTQIIPSK TQGNPEYLIM NINTPSTATI 840
GPHKRAEQSY EVSIRKPFDV EDSPILAIHF KCGGSTNTIN LKTAIGMTTT LISSDVNPSM 900
HLNLAQFISR WKTLSDALGK EGEYQKSGIK LNKDFRKVET ISLEDGLLLL TQTVKRLGFD 960
IVDQTSVRST LFVSGIIHTK SEGNFGCLMK IQYQVNGTVN VTCKTTTAGP LAKYIVECIK 1020
NVLTK 1025 
Gene Ontology
 GO:0030122; C:AP-2 adaptor complex; IMP:SGD.
 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0008565; F:protein transporter activity; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IC:SGD.
 GO:0016192; P:vesicle-mediated transport; IEA:InterPro. 
Interpro
 IPR017104; AP2_complex_asu.
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR002553; Clathrin/coatomer_adapt-like_N.
 IPR013038; Clathrin_a-adaptin_app_Ig-like.
 IPR008152; Clathrin_a/b/g-adaptin_app_Ig.
 IPR015873; Clathrin_a/coatomer_app_sub_C.
 IPR009028; Coatomer/calthrin_app_sub_C.
 IPR013041; Coatomer/clathrin_app_Ig-like. 
Pfam
 PF01602; Adaptin_N
 PF02883; Alpha_adaptinC2 
SMART
  
PROSITE
  
PRINTS