CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015147
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like 
Protein Synonyms/Alias
 tRNA-dihydrouridine synthase 1-like 
Gene Name
 DUS1L 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
139MILLAHEKLSVPVTCubiquitination[1]
147LSVPVTCKIRVFPEIubiquitination[1]
156RVFPEIDKTVRYAQMubiquitination[1]
166RYAQMLEKAGCQLLTubiquitination[1]
195AASWEHIKAVRKAVAubiquitination[1]
309AAVSQELKLRCQEEIubiquitination[1]
323ISRQEGAKPTGDLPFubiquitination[1]
372TEVLSKNKQKKQLRNubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs (By similarity). 
Sequence Annotation
  
Keyword
 Complete proteome; FAD; Flavoprotein; Oxidoreductase; Reference proteome; tRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 473 AA 
Protein Sequence
MPKLQGFEFW SRTLRGARHV VAPMVDQSEL AWRLLSRRHG AQLCYTPMLH AQVFVRDANY 60
RKENLYCEVC PEDRPLIVQF CANDPEVFVQ AALLAQDYCD AIDLNLGCPQ MIAKRGHYGA 120
FLQDEWDLLQ RMILLAHEKL SVPVTCKIRV FPEIDKTVRY AQMLEKAGCQ LLTVHGRTKE 180
QKGPLSGAAS WEHIKAVRKA VAIPVFANGN IQCLQDVERC LRDTGVQGVM SAEGNLHNPA 240
LFEGRSPAVW ELAEEYLDIV REHPCPLSYV RAHLFKLWHH TLQVHQELRE ELAKVKTLEG 300
IAAVSQELKL RCQEEISRQE GAKPTGDLPF HWICQPYIRP GPREGSKEKA GARSKRALEE 360
EEGGTEVLSK NKQKKQLRNP HKTFDPSLKP KYAKCDQCGN PKGNRCVFSL CRGCCKKRAS 420
KETADCPGHG LLFKTKLEKS LAWKEAQPEL QEPQPAAPGT PGGFSEVMGS ALA 473 
Gene Ontology
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0017150; F:tRNA dihydrouridine synthase activity; IEA:InterPro.
 GO:0002943; P:tRNA dihydrouridine synthesis; IEA:GOC. 
Interpro
 IPR013785; Aldolase_TIM.
 IPR001269; tRNA_hU_synthase.
 IPR018517; tRNA_hU_synthase_CS. 
Pfam
 PF01207; Dus 
SMART
  
PROSITE
 PS01136; UPF0034 
PRINTS