CPLM-019559

Genbank Nucleotide ID

Sodium-coupled neutral amino acid transporter 2

Protein Synonyms/Alias

Amino acid transporter A2; Protein 40-9-1; Solute carrier family 38 member 2; System A amino acid transporter 2; System A transporter 1; System N amino acid transporter 2

ATA2; KIAA1382; SAT2; SNAT2

Homo sapiens (Human)

Position	Peptide	Type	References
2	******MKKAEMGRF	ubiquitination	[1]
3	*****MKKAEMGRFS	ubiquitination	[1, 2]
33	FNYSYPTKQAALKSH	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
38	PTKQAALKSHYADVD	ubiquitination	[1, 2, 3, 4, 6, 7]
59	LLESNLGKKKYETEF	ubiquitination	[1, 2, 4, 6, 7, 8, 9]
60	LESNLGKKKYETEFH	ubiquitination	[1, 2, 6]
140	LYEQLGYKAFGLVGK	ubiquitination	[2, 6, 10]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[8] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[9] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[10] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]

Functional Description

Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of neutral amino acids and sodium ions with a stoichiometry of 1:1. May function in the transport of amino acids at the blood- brain barrier and in the supply of maternal nutrients to the fetus through the placenta.

REGION 1 96 Regulates protein turnover upon amino
MOD_RES 10 10 Phosphoserine.
MOD_RES 12 12 Phosphoserine.
MOD_RES 22 22 Phosphoserine.
MOD_RES 41 41 Phosphotyrosine (By similarity).
MOD_RES 55 55 Phosphoserine (By similarity).
CARBOHYD 258 258 N-linked (GlcNAc...) (Potential).
CARBOHYD 274 274 N-linked (GlcNAc...) (Potential).

Alternative splicing; Amino-acid transport; Cell membrane; Complete proteome; Glycoprotein; Ion transport; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix; Transport; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0005886; C:plasma membrane; TAS:Reactome.
GO:0015171; F:amino acid transmembrane transporter activity; IEA:Compara.
GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
GO:0014047; P:glutamate secretion; TAS:Reactome.
GO:0007269; P:neurotransmitter secretion; TAS:Reactome.
GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
GO:0055085; P:transmembrane transport; TAS:Reactome.

IPR013057; AA_transpt_TM.