CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015846
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase SHPRH 
Protein Synonyms/Alias
 SNF2, histone-linker, PHD and RING finger domain-containing helicase 
Gene Name
 Shprh 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
518EDVFDKTKKQAVGSPacetylation[1]
1553FTQISRIKTFQENLSubiquitination[2]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63' (By similarity). 
Sequence Annotation
 DOMAIN 302 384 Helicase ATP-binding; first part.
 DOMAIN 433 507 H15.
 DOMAIN 701 859 Helicase ATP-binding; second part.
 DOMAIN 1505 1663 Helicase C-terminal.
 NP_BIND 368 375 ATP (By similarity).
 ZN_FING 649 700 PHD-type.
 ZN_FING 1423 1470 RING-type.
 MOTIF 810 813 DEAQ box.
 MOD_RES 261 261 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; DNA damage; DNA repair; Helicase; Hydrolase; Ligase; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1674 AA 
Protein Sequence
MSSRRKRAPP MKVDEERQQQ LHWNMHEDLR SEPLTMTVGE QACSDADSSS DCIIIDEGPP 60
ESALHRDKKR RSETVSVLEA TEEETRLSVT LNVTVSPYRV DNSWKAFLGD FALQLLPKES 120
LVEHFSERTF TLSPSESSSQ FLIYVHSECK NVEKQENVLE GSAGVCSKGI RVESSFSSDM 180
LQDLAWLQKR RGIKLYQRPD GTHTIKVGIY ILEAGLTRLD FMSDAGSRMK KFNQLMKRVM 240
EKLHNFIIPD VLEEEEEGSE SEPEGQDIDE LYHFVKQTHQ QETRSVQVDV QHPALIPVLR 300
PYQREAVNWM LQQEQFRSAP PADNSLHFLW REIVTPDGLK LYYNPYTGCI IRDFPHAGPQ 360
LLGGILADEM GLGKTVEVLA LILTHTRQDV KQDALTLPEG KVVNYFIPTH CPREKVKNRE 420
IQDTEYEPKE KVHCPPTRVM ILTAVKEMNG KKGVSILSIY KYVSSIFRYD VQRNRGLLKR 480
MLKCLIFEGL VKQIKGHGFS GTFTLGKNYK EDVFDKTKKQ AVGSPRKIEK ELRKSVNKDA 540
DSEYLPSNTS DDDEPYYYYC KAGKSRSKLK KPALLTKKGK GQSVHLDSQG DAPAAGVCAS 600
TDVHVSENTC VSEDKQTQEA KDCAESPNPA AEELAQSNTS SPCETSDYRF ECICGEFDQI 660
GHKPRVQCLK CHLWQHAKCV NYEEKNLKVK PFYCPHCLVA MEPVSTRATL IISPSSICHQ 720
WVDEINRHVR SSSLRVLVYQ GVKKHGFLQP HFLAEQDIVI ITYDVLRSEL NYVNIPHSNS 780
EDGRRLRNQK RYMAIPSPLV AVEWWRICLD EAQMVECPTV KAAEMAQRLS GINRWCISGT 840
PVQRGLEDLF GLVVFLGIEP YCVKHWWIRL LYHPYCKKNP QHLYSFIAKI MWRSAKKDVI 900
DQIQIPPQTE EMHWLHFSPV ERHFYHRQHE VCCQDAIVKL RKISDWALKL SSLDRRTVSS 960
ILYPLLRLRQ ACCHPQAVRG EFLPLQKSTM TMEELLTSLQ KKCGTECEEA HRQLVCALNG 1020
LAGIHIIKGE YALAAELYRE VLRSSEEHKG KLKTDSLQRL HATHNLMELL GAKHPGIPPT 1080
LRDGRLEEEA KQLREHYMSK CNTEVAEAQQ ALQPVQQSIR ELQRKIHSNS PWWLNVIHRA 1140
MEFSVDEELV QRVRNEISSN YKQQTDKLSM SEKFRDCRGL QFLLTTQMEE LHKFQKLVRE 1200
AVKKLEKPPS REVIESATVC HLRPARLPLN CCVFCKADEL FTEYESKLFF NTVKGQTAIF 1260
EEMIEDEEGL VDDRVPTTTR GLWAVSETER SMKAILSFAR SHRFDVEYVD EGSVSMDLFE 1320
AWKKEYKLLH EYWMTLRNRV SAVDELAMAT ERLRVRHPKE PKPNPPVHHI IEPHEVEQNR 1380
IKLVNDKAVA TSQLQKKLGQ LLYLTNLEKS QDKTSGGINP EPCPICARQL GKQWAVLTCG 1440
HCFCNECTSI IIEQYSVGSH RSSIKCAICR QTTSHKEVSY VFTSEKANQE DDIPVKGSHS 1500
TKVEAVVRTL MKIQLRDPGA KALVFSTWQD VLDIISKALT DNNMEFTQIS RIKTFQENLS 1560
AFKYDPHINI LLLPLHTGSN GLTIIEATHV LLVEPILNPA HELQAIGRVH RIGQTKPTIV 1620
HRFLIKATIE ERMQAMLKTA ERSHTSSSGK HSEASVLTVA GLADLFTKEN EELE 1674 
Gene Ontology
 GO:0000786; C:nucleosome; IEA:InterPro.
 GO:0005634; C:nucleus; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0006334; P:nucleosome assembly; IEA:InterPro.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway. 
Interpro
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR005818; Histone_H1/H5.
 IPR027417; P-loop_NTPase.
 IPR000330; SNF2_N.
 IPR011991; WHTH_DNA-bd_dom.
 IPR019786; Zinc_finger_PHD-type_CS.
 IPR011011; Znf_FYVE_PHD.
 IPR001965; Znf_PHD.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF00271; Helicase_C
 PF00538; Linker_histone
 PF00176; SNF2_N 
SMART
 SM00487; DEXDc
 SM00526; H15
 SM00249; PHD
 SM00184; RING 
PROSITE
 PS51504; H15
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS01359; ZF_PHD_1
 PS50016; ZF_PHD_2
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS