CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017137
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein-associating with the carboxyl-terminal domain of ezrin 
Protein Synonyms/Alias
 Ezrin-binding protein PACE-1; SCY1-like protein 3 
Gene Name
 SCYL3 
Gene Synonyms/Alias
 PACE1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
272EEKTEFFKFLLDRVSubiquitination[1, 2, 3, 4, 5]
500DPFSQPIKFPINGLSubiquitination[2]
524ENFPSSSKKSEEWPDubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 May play a role in regulating cell adhesion/migration complexes in migrating cells. 
Sequence Annotation
 DOMAIN 2 245 Protein kinase.
 REPEAT 199 238 HEAT 1.
 REPEAT 285 323 HEAT 2.
 REPEAT 333 370 HEAT 3.
 REPEAT 372 409 HEAT 4.
 REGION 548 742 Interaction with EZR.
 MOD_RES 476 476 Phosphoserine (By similarity).
 LIPID 2 2 N-myristoyl glycine (Probable).  
Keyword
 Alternative splicing; Cell projection; Complete proteome; Cytoplasm; Golgi apparatus; Lipoprotein; Myristate; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 742 AA 
Protein Sequence
MGSENSALKS YTLREPPFTL PSGLAVYPAV LQDGKFASVF VYKRENEDKV NKAAKHLKTL 60
RHPCLLRFLS CTVEADGIHL VTERVQPLEV ALETLSSAEV CAGIYDILLA LIFLHDRGHL 120
THNNVCLSSV FVSEDGHWKL GGMETVCKVS QATPEFLRSI QSIRDPASIP PEEMSPEFTT 180
LPECHGHARD AFSFGTLVES LLTILNEQVS ADVLSSFQQT LHSTLLNPIP KCRPALCTLL 240
SHDFFRNDFL EVVNFLKSLT LKSEEEKTEF FKFLLDRVSC LSEELIASRL VPLLLNQLVF 300
AEPVAVKSFL PYLLGPKKDH AQGETPCLLS PALFQSRVIP VLLQLFEVHE EHVRMVLLSH 360
IEAYVEHFTQ EQLKKVILPQ VLLGLRDTSD SIVAITLHSL AVLVSLLGPE VVVGGERTKI 420
FKRTAPSFTK NTDLSLEDSP MCVVCSHHSQ ISPILENPFS SIFPKCFFSG STPINSKKHI 480
QRDYYNTLLQ TGDPFSQPIK FPINGLSDVK NTSEDSENFP SSSKKSEEWP DWSEPEEPEN 540
QTVNIQIWPR EPCDDVKSQC TTLDVEESSW DDCEPSSLDT KVNPGGGITA TKPVTSGEQK 600
PIPALLSLTE ESMPWKSSLP QKISLVQRGD DADQIEPPKV SSQERPLKVP SELGLGEEFT 660
IQVKKKPVKD PEMDWFADMI PEIKPSAAFL ILPELRTEMV PKKDDVSPVM QFSSKFAAAE 720
ITEGEAEGWE EEGELNWEDN NW 742 
Gene Ontology
 GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
 GO:0030027; C:lamellipodium; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0016772; F:transferase activity, transferring phosphorus-containing groups; IEA:InterPro.
 GO:0016477; P:cell migration; NAS:UniProtKB.
 GO:0006468; P:protein phosphorylation; IEA:InterPro. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR021133; HEAT_type_2.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT
 PS50011; PROTEIN_KINASE_DOM 
PRINTS