UniProt Accession

 MP2K7_MOUSE; Q8CE90; O35406; O35720; O35871; O35872; O54780; O70242; O70243; Q8BSP1; Q9QWG6; Q9R1Z3; Q9R1Z4; Q9R1Z5; Q9R1Z6 

Genbank Protein ID

 AF026216; U74463; U74464; AB005654; AF022112; AF022113; U93030; U93031; AF060943; AF060944; AF060945; AF060946; AF060947; AK028772; AK031137; AK165184; BC070467 

Genbank Nucleotide ID

 AAB81848.1; AAC53364.1; AAC53365.1; BAA24383.1; AAC16274.1; AAC16275.1; AAB63447.1; AAB63448.1; AAD15819.1; AAD15820.1; AAD15821.1; AAD15822.1; AAD15823.1; BAC26111.1; BAC27272.1; BAE38066.1; AAH70467.1 

Protein Name

 Dual specificity mitogen-activated protein kinase kinase 7 

Protein Synonyms/Alias

 MAP kinase kinase 7; MAPKK 7; JNK-activating kinase 2; MAPK/ERK kinase 7; MEK 7; c-Jun N-terminal kinase kinase 2; JNK kinase 2; JNKK 2 

Gene Name

 Map2k7 

Gene Synonyms/Alias

 Mkk7 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
9	AASSLEQKLSRLEAK	ubiquitination	[1]
342	QFPYKNCKTDFEVLT	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. 

Sequence Annotation

 DOMAIN 136 396 Protein kinase.
 NP_BIND 142 150 ATP (By similarity).
 REGION 37 73 D domain (By similarity).
 REGION 393 416 DVD domain (By similarity).
 ACT_SITE 259 259 Proton acceptor (By similarity).
 BINDING 165 165 ATP (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 287 287 Phosphoserine; by MAP3K (By similarity).
 MOD_RES 291 291 Phosphothreonine; by MAP3K (By  

Keyword

 Acetylation; Alternative splicing; Apoptosis; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Stress response; Transferase; Tyrosine-protein kinase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 535 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008545; F:JUN kinase kinase activity; IEA:Compara.
 GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
 GO:0004708; F:MAP kinase kinase activity; IDA:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0004713; F:protein tyrosine kinase activity; IEA:UniProtKB-KW.
 GO:0007257; P:activation of JUN kinase activity; IDA:BHF-UCL.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0043525; P:positive regulation of neuron apoptotic process; IEA:Compara. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

 SM00220; S_TKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS