CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004177
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Electron transfer flavoprotein subunit alpha, mitochondrial 
Protein Synonyms/Alias
 Alpha-ETF 
Gene Name
 ETFA 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
85VAQHDVYKGLLPEELacetylation[1]
85VAQHDVYKGLLPEELubiquitination[2, 3, 4, 5]
126LLPRVAAKLEVAPISubiquitination[3, 4]
139ISDIIAIKSPDTFVRubiquitination[2, 3, 4, 5, 6]
164VKCDEKVKVFSVRGTubiquitination[6]
187GGSASSEKASSTSPVubiquitination[4]
203ISEWLDQKLTKSDRPubiquitination[4, 6]
206WLDQKLTKSDRPELTubiquitination[4]
216RPELTGAKVVVSGGRubiquitination[2, 3, 4, 5]
226VSGGRGLKSGENFKLubiquitination[2, 4, 5]
232LKSGENFKLLYDLADubiquitination[4]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
 The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl- CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). 
Sequence Annotation
 NP_BIND 274 302 FAD (Potential).  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Direct protein sequencing; Disease mutation; Electron transport; FAD; Flavoprotein; Glutaricaciduria; Mitochondrion; Polymorphism; Reference proteome; Transit peptide; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 333 AA 
Protein Sequence
MFRAAAPGQL RRAASLLRFQ STLVIAEHAN DSLAPITLNT ITAATRLGGE VSCLVAGTKC 60
DKVAQDLCKV AGIAKVLVAQ HDVYKGLLPE ELTPLILATQ KQFNYTHICA GASAFGKNLL 120
PRVAAKLEVA PISDIIAIKS PDTFVRTIYA GNALCTVKCD EKVKVFSVRG TSFDAAATSG 180
GSASSEKASS TSPVEISEWL DQKLTKSDRP ELTGAKVVVS GGRGLKSGEN FKLLYDLADQ 240
LHAAVGASRA AVDAGFVPND MQVGQTGKIV APELYIAVGI SGAIQHLAGM KDSKTIVAIN 300
KDPEAPIFQV ADYGIVADLF KVVPEMTEIL KKK 333 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0009055; F:electron carrier activity; IDA:UniProtKB.
 GO:0050660; F:flavin adenine dinucleotide binding; IDA:UniProtKB.
 GO:0016491; F:oxidoreductase activity; IDA:UniProtKB.
 GO:0022904; P:respiratory electron transport chain; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR001308; ETF_a.
 IPR014730; ETF_a/b_N.
 IPR014731; ETF_asu_C.
 IPR018206; ETF_asu_C_CS.
 IPR014729; Rossmann-like_a/b/a_fold. 
Pfam
 PF01012; ETF
 PF00766; ETF_alpha 
SMART
 SM00893; ETF 
PROSITE
 PS00696; ETF_ALPHA 
PRINTS