CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017872
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 N-acetylglutamate synthase, mitochondrial 
Protein Synonyms/Alias
 Amino-acid acetyltransferase; N-acetylglutamate synthase long form; N-acetylglutamate synthase short form; N-acetylglutamate synthase conserved domain form 
Gene Name
 Nags 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
282AKALQPTKIIFLNNSubiquitination[1]
480PINPWYFKHSDGSFSubiquitination[1]
520GLPDSFCKPASDPGSacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 
Functional Description
 Plays a role in the regulation of ureagenesis by producing variable amounts of N-acetylglutamate (NAG), thus modulating carbamoylphosphate synthase I (CPSI) activity. 
Sequence Annotation
 DOMAIN 371 515 N-acetyltransferase.  
Keyword
 Acyltransferase; Alternative splicing; Complete proteome; Direct protein sequencing; Mitochondrion; Reference proteome; Transferase; Transit peptide; Urea cycle. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 527 AA 
Protein Sequence
MATAWVATAL RSAAAARRLR SPGGPGGSRR LSGSARRRGA KSASPGRRLS TARAHAEDAE 60
GAKGRVQSPA VEEPSWTPLP TPLESPAPPA GRSLVQRDIQ AFLNQCGASP GEARHWLTQF 120
QTCYHSVDKP FAVMEVDEEV IRCPQAVSRL AFALAFLQRM DMKPLVVLGL PTPTAPSGCL 180
SFWEAKAQLA QSCKVLVDEL RHNAATAVPF FGGGSVLSAA EPAPHASYGG IVAVETDLLQ 240
WCLESNSIPI LCPIGETAAR RSVLLDSLEV TASLAKALQP TKIIFLNNSG GLRNNSQKIL 300
SNVNLPADLD LVTNAEWLSI KERQQIRLIV DVLSRLPHYS SAVITAASTL LTELFSNKGC 360
GTLFKNAERM LRVRNLDSLD QGRLVNLVNA SFGKKLREDY LESLRPRLHS IYVSEGYNAA 420
AILTVEPVLG GTPYLDKFVV SSSRQGQGSG QMLWECLRRD LQTLFWRSRV TNPINPWYFK 480
HSDGSFSNKQ WIFFWFGLAD IRDSYELVNH AKGLPDSFCK PASDPGS 527 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0004042; F:acetyl-CoA:L-glutamate N-acetyltransferase activity; IDA:MGI.
 GO:0006526; P:arginine biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006536; P:glutamate metabolic process; IDA:MGI.
 GO:0000050; P:urea cycle; IEA:UniProtKB-KW. 
Interpro
 IPR016181; Acyl_CoA_acyltransferase.
 IPR001048; Asp/Glu/Uridylate_kinase.
 IPR006855; DUF619.
 IPR011243; GlcNAc_Synth_met.
 IPR000182; GNAT_dom. 
Pfam
 PF04768; DUF619 
SMART
  
PROSITE
 PS51186; GNAT 
PRINTS