UniProt Accession

 PCNA_MOUSE; P17918 

Genbank Protein ID

 X53068; X57800; BC005778; BC010343 

Genbank Nucleotide ID

 CAA37243.1; CAA40938.1; AAH05778.1; AAH10343.1 

Protein Name

 Proliferating cell nuclear antigen 

Protein Synonyms/Alias

 PCNA; Cyclin 

Gene Name

 Pcna 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
80	TSMSKILKCAGNEDI	ubiquitination	[1, 2]
164	AVVISCAKNGVKFSA	ubiquitination	[1]
248	IADMGHLKYYLAPKI	ubiquitination	[1, 2]
254	LKYYLAPKIEDEEAS	ubiquitination	[2]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs.
 Mathew R, Seiler MP, Scanlon ST, Mao AP, Constantinides MG, Bertozzi-Villa C, Singer JD, Bendelac A.
 Nature. 2012 Nov 22;491(7425):618-21. [PMID: 23086144] 

Functional Description

 Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (By similarity). 

Sequence Annotation

 DNA_BIND 61 80 Potential.
 MOD_RES 14 14 N6-acetyllysine (By similarity).
 MOD_RES 77 77 N6-acetyllysine (By similarity).
 MOD_RES 80 80 N6-acetyllysine (By similarity).
 MOD_RES 211 211 Phosphotyrosine; by EGFR.
 MOD_RES 248 248 N6-acetyllysine (By similarity).
 CROSSLNK 164 164 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 261 AA 

Protein Sequence

Gene Ontology

 GO:0000307; C:cyclin-dependent protein kinase holoenzyme complex; IPI:MGI.
 GO:0005652; C:nuclear lamina; IDA:MGI.
 GO:0043626; C:PCNA complex; IEA:InterPro.
 GO:0070557; C:PCNA-p21 complex; ISS:UniProtKB.
 GO:0005657; C:replication fork; IDA:MGI.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0030337; F:DNA polymerase processivity factor activity; IEA:InterPro.
 GO:0006287; P:base-excision repair, gap-filling; IDA:MGI.
 GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0032077; P:positive regulation of deoxyribonuclease activity; ISS:UniProtKB.
 GO:0006275; P:regulation of DNA replication; IEA:InterPro.
 GO:0019985; P:translesion synthesis; ISS:UniProtKB. 

Interpro

 IPR000730; Pr_cel_nuc_antig.
 IPR022649; Pr_cel_nuc_antig_C.
 IPR022659; Pr_cel_nuc_antig_CS.
 IPR022648; Pr_cel_nuc_antig_N. 

Pfam

 PF02747; PCNA_C
 PF00705; PCNA_N 

SMART

  

PROSITE

 PS01251; PCNA_1
 PS00293; PCNA_2 

PRINTS

 PR00339; PCNACYCLIN.