UniProt Accession

 KTNA1_HUMAN; O75449; E1P5A3; Q5TFA8; Q5TFA9; Q86VN2; Q9NU52 

Genbank Protein ID

 AF056022; AL078581; BX276089; AL078581; BX276089; BX276089; AL078581; BX276089; AL078581; CH471051; CH471051; BC050428 

Genbank Nucleotide ID

 AAC25114.1; CAI19504.1; CAI19504.1; CAI19505.1; CAI19505.1; CAI16431.1; CAI16431.1; CAI16432.1; CAI16432.1; EAW47793.1; EAW47795.1; AAH50428.1 

Protein Name

 Katanin p60 ATPase-containing subunit A1 

Protein Synonyms/Alias

 Katanin p60 subunit A1; p60 katanin 

Gene Name

 KATNA1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
51	KDTYLQQKWQQVWQE	ubiquitination	[1]
71	KHVKDIMKTLESFKL	ubiquitination	[2, 3]
181	VTEPETNKFDSTGYD	ubiquitination	[1]
218	IADLVEAKKLLKEAV	ubiquitination	[1]
219	ADLVEAKKLLKEAVV	ubiquitination	[4]
236	MWMPEFFKGIRRPWK	ubiquitination	[4]
281	SSSTLTSKYRGESEK	ubiquitination	[2, 3]
385	YIPLPSAKGREELLR	ubiquitination	[4]
450	EEIRNLSKEEMHMPT	ubiquitination	[4]
471	MALKKVSKSVSAADI	ubiquitination	[1]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth (By similarity). 

Sequence Annotation

 NP_BIND 249 256 ATP (Potential).
 REGION 1 185 Interaction with microtubules.
 REGION 1 75 Interaction with dynein and NDEL1 (By
 REGION 1 29 Interaction with KATNB1.
 MOD_RES 42 42 Phosphoserine; by DYRK2.
 MOD_RES 109 109 Phosphoserine; by DYRK2.
 MOD_RES 133 133 Phosphothreonine; by DYRK2.
 MOD_RES 170 170 Phosphoserine.  

Keyword

 Alternative splicing; ATP-binding; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Hydrolase; Microtubule; Mitosis; Nucleotide-binding; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 491 AA 

Protein Sequence

Gene Ontology

 GO:0030424; C:axon; IEA:Compara.
 GO:0005813; C:centrosome; IDA:HPA.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0030426; C:growth cone; IEA:Compara.
 GO:0005811; C:lipid particle; IEA:Compara.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0030496; C:midbody; IDA:UniProtKB.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0000922; C:spindle pole; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008017; F:microtubule binding; IDA:UniProtKB.
 GO:0008568; F:microtubule-severing ATPase activity; IEA:EC.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0031122; P:cytoplasmic microtubule organization; IEA:Compara.
 GO:0051329; P:interphase of mitotic cell cycle; IMP:UniProtKB.
 GO:0001578; P:microtubule bundle formation; IEA:Compara.
 GO:0051013; P:microtubule severing; IEA:Compara.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0010977; P:negative regulation of neuron projection development; IEA:Compara.
 GO:0001764; P:neuron migration; IEA:Compara.
 GO:0008104; P:protein localization; IEA:Compara. 

Interpro

 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR003960; ATPase_AAA_CS.
 IPR027417; P-loop_NTPase.
 IPR015415; Vps4_C. 

Pfam

 PF00004; AAA
 PF09336; Vps4_C 

SMART

 SM00382; AAA 

PROSITE

 PS00674; AAA 

PRINTS