CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010420
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 mRNA export factor 
Protein Synonyms/Alias
 Rae1 protein homolog; mRNA-associated protein mrnp 41 
Gene Name
 RAE1 
Gene Synonyms/Alias
 MRNP41 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
80DSGQTIPKAQQMHTGubiquitination[1]
100CWSDDGSKVFTASCDubiquitination[1]
108VFTASCDKTAKMWDLubiquitination[1]
111ASCDKTAKMWDLSSNubiquitination[1, 2, 3]
131AQHDAPVKTIHWIKAubiquitination[1]
151VMTGSWDKTLKFWDTubiquitination[1]
154GSWDKTLKFWDTRSSubiquitination[1, 2, 3, 4]
212RRIESPLKHQHRCVAubiquitination[1]
222HRCVAIFKDKQNKPTubiquitination[1]
224CVAIFKDKQNKPTGFubiquitination[1, 3]
227IFKDKQNKPTGFALGubiquitination[2]
252INPPNPAKDNFTFKCubiquitination[1, 2, 3, 5]
258AKDNFTFKCHRSNGTubiquitination[1]
302GRFSFWDKDARTKLKubiquitination[1, 2, 3]
309KDARTKLKTSEQLDQubiquitination[1, 6]
340ASSYDWSKGHEFYNPubiquitination[1]
350EFYNPQKKNYIFLRNubiquitination[1, 7]
363RNAAEELKPRNKK**ubiquitination[1, 7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Binds mRNA. May function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. 
Sequence Annotation
 REPEAT 37 79 WD 1.
 REPEAT 84 114 WD 2.
 REPEAT 125 157 WD 3.
 REPEAT 168 206 WD 4.
 REPEAT 215 255 WD 5.
 REPEAT 271 301 WD 6.
 REPEAT 310 346 WD 7.
 MOD_RES 229 229 Phosphothreonine.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 368 AA 
Protein Sequence
MSLFGTTSGF GTSGTSMFGS ATTDNHNPMK DIEVTSSPDD SIGCLSFSPP TLPGNFLIAG 60
SWANDVRCWE VQDSGQTIPK AQQMHTGPVL DVCWSDDGSK VFTASCDKTA KMWDLSSNQA 120
IQIAQHDAPV KTIHWIKAPN YSCVMTGSWD KTLKFWDTRS SNPMMVLQLP ERCYCADVIY 180
PMAVVATAER GLIVYQLENQ PSEFRRIESP LKHQHRCVAI FKDKQNKPTG FALGSIEGRV 240
AIHYINPPNP AKDNFTFKCH RSNGTNTSAP QDIYAVNGIA FHPVHGTLAT VGSDGRFSFW 300
DKDARTKLKT SEQLDQPISA CCFNHNGNIF AYASSYDWSK GHEFYNPQKK NYIFLRNAAE 360
ELKPRNKK 368 
Gene Ontology
 GO:0005737; C:cytoplasm; TAS:ProtInc.
 GO:0005856; C:cytoskeleton; TAS:ProtInc.
 GO:0005640; C:nuclear outer membrane; TAS:ProtInc.
 GO:0005643; C:nuclear pore; TAS:ProtInc.
 GO:0008017; F:microtubule binding; ISS:UniProtKB.
 GO:0003723; F:RNA binding; TAS:ProtInc.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0071407; P:cellular response to organic cyclic compound; IEA:Compara.
 GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
 GO:0015758; P:glucose transport; TAS:Reactome.
 GO:0006406; P:mRNA export from nucleus; TAS:ProtInc.
 GO:0010827; P:regulation of glucose transport; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0055085; P:transmembrane transport; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 
Interpro
 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.