| Tag | Content |
|---|
| CPLM ID | CPLM-010200 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Pyrimidine monooxygenase RutA |
Protein Synonyms/Alias | |
Gene Name | rutA |
Gene Synonyms/Alias | ycdM; b1012; JW0997 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 296 | WLTEQSQKDTRSGTD | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Catalyzes the pyrimidine ring opening between N-3 and C- 4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product which can be spontaneously reduced to ureidoacrylate. Requires the flavin reductase RutF to regenerate FMN in vivo. RutF can be substituted by Fre in vitro. |
Sequence Annotation | REGION 68 69 FMN binding (Potential).
REGION 159 160 FMN binding (Potential).
BINDING 134 134 FMN (Potential).
BINDING 143 143 FMN (Potential).
BINDING 209 209 FMN (Potential). |
Keyword | Complete proteome; FMN; Monooxygenase; NADP; Oxidoreductase; Reference proteome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 382 AA |
Protein Sequence | MQDAAPRLTF TLRDEERLMM KIGVFVPIGN NGWLISTHAP QYMPTFELNK AIVQKAEHYH 60
FDFALSMIKL RGFGGKTEFW DHNLESFTLM AGLAAVTSRI QIYATAATLT LPPAIVARMA 120
ATIDSISGGR FGVNLVTGWQ KPEYEQMGIW PGDDYFSRRY DYLTEYVQVL RDLWGTGKSD 180
FKGDFFTMND CRVSPQPSVP MKVICAGQSD AGMAFSARYA DFNFCFGKGV NTPTAFAPTA 240
ARMKQAAEQT GRDVGSYVLF MVIADETDDA ARAKWEHYKA GADEEALSWL TEQSQKDTRS 300
GTDTNVRQMA DPTSAVNINM GTLVGSYASV ARMLDEVASV PGAEGVLLTF DDFLSGIETF 360
GERIQPLMQC RAHLPALTQE VA 382 |
Gene Ontology | GO:0004497; F:monooxygenase activity; IDA:UniProtKB. GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro. GO:0052614; F:uracil oxygenase activity; IDA:EcoCyc. GO:0019740; P:nitrogen utilization; IDA:UniProtKB. GO:0006212; P:uracil catabolic process; IDA:UniProtKB. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |