| Tag | Content |
|---|
| CPLM ID | CPLM-019054 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | E3 ubiquitin-protein ligase MIB2 |
Protein Synonyms/Alias | Mind bomb homolog 2; Novel zinc finger protein; Novelzin; Putative NF-kappa-B-activating protein 002N; Skeletrophin; Zinc finger ZZ type with ankyrin repeat domain protein 1 |
Gene Name | MIB2 |
Gene Synonyms/Alias | SKD; ZZANK1 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 80 | VVRGVDWKWGQQDGG | ubiquitination | [1] | | 220 | RGIFQGAKVVRGPDW | ubiquitination | [1] | | 320 | QPFQHGDKVKCLLDT | ubiquitination | [1] | | 322 | FQHGDKVKCLLDTDV | ubiquitination | [1, 2] | | 402 | IGDLDTVKRLQAGHG | ubiquitination | [1] | | 427 | GRVGKVVKVFGDGNL | ubiquitination | [1, 2] | | 480 | SLSVALDKLRAQKSD | ubiquitination | [1] | | 689 | RQLVDAKKEDGFTAL | ubiquitination | [1] | | 842 | AAEGRVLKALQGCAQ | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles. Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C. Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [ PMID: 21890473] |
Functional Description | E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors (By similarity). |
Sequence Annotation | DOMAIN 59 138 MIB/HERC2 1.
DOMAIN 207 285 MIB/HERC2 2.
REPEAT 522 551 ANK 1.
REPEAT 555 584 ANK 2.
REPEAT 588 617 ANK 3.
REPEAT 621 653 ANK 4.
REPEAT 657 686 ANK 5.
REPEAT 691 721 ANK 6.
REPEAT 725 754 ANK 7.
REPEAT 758 786 ANK 8.
REPEAT 827 856 ANK 9.
ZN_FING 143 190 ZZ-type.
ZN_FING 890 925 RING-type 1.
ZN_FING 969 1002 RING-type 2.
MOD_RES 309 309 Phosphoserine (By similarity). |
Keyword | Actin-binding; Alternative splicing; ANK repeat; Complete proteome; Cytoplasm; Endosome; Ligase; Metal-binding; Notch signaling pathway; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1013 AA |
Protein Sequence | MGWKPSEARG QSQSFQASGL QPRSLKAARR ATGRPDRSRA ARPTMDPSAH RSRAAPPNMD 60
PDPQAGVQVG MRVVRGVDWK WGQQDGGEGG VGTVVELGRH GSPSTPDRTV VVQWDQGTRT 120
NYRAGYQGAH DLLLYDNAQI GVRHPNIICD CCKKHGLRGM RWKCRVCLDY DLCTQCYMHN 180
KHELAHAFDR YETAHSRPVT LSPRQGLPRI PLRGIFQGAK VVRGPDWEWG SQDGGEGKPG 240
RVVDIRGWDV ETGRSVASVT WADGTTNVYR VGHKGKVDLK CVGEAAGGFY YKDHLPRLGK 300
PAELQRRVSA DSQPFQHGDK VKCLLDTDVL REMQEGHGGW NPRMAEFIGQ TGTVHRITDR 360
GDVRVQFNHE TRWTFHPGAL TKHHSFWVGD VVRVIGDLDT VKRLQAGHGE WTDDMAPALG 420
RVGKVVKVFG DGNLRVAVAG QRWTFSPSCL VAYRPEEDAN LDVAERAREN KSSLSVALDK 480
LRAQKSDPEH PGRLVVEVAL GNAARALDLL RRRPEQVDTK NQGRTALQVA AYLGQVELIR 540
LLLQARAGVD LPDDEGNTAL HYAALGNQPE ATRVLLSAGC RADAINSTQS TALHVAVQRG 600
FLEVVRALCE RGCDVNLPDA HSDTPLHSAI SAGTGASGIV EVLTEVPNID VTATNSQGFT 660
LLHHASLKGH ALAVRKILAR ARQLVDAKKE DGFTALHLAA LNNHREVAQI LIREGRCDVN 720
VRNRKLQSPL HLAVQQAHVG LVPLLVDAGC SVNAEDEEGD TALHVALQRH QLLPLVADGA 780
GGDPGPLQLL SRLQASGLPG SAELTVGAAV ACFLALEGAD VSYTNHRGRS PLDLAAEGRV 840
LKALQGCAQR FRERQAGGGA APGPRQTLGT PNTVTNLHVG AAPGPEAAEC LVCSELALLV 900
LFSPCQHRTV CEECARRMKK CIRCQVVVSK KLRPDGSEVA SAAPAPGPPR QLVEELQSRY 960
RQMEERITCP ICIDSHIRLV FQCGHGACAP CGSALSACPI CRQPIRDRIQ IFV 1013 |
Gene Ontology | GO:0005768; C:endosome; IEA:UniProtKB-SubCell. GO:0004871; F:signal transducer activity; IMP:UniProtKB. GO:0004842; F:ubiquitin-protein ligase activity; IEA:InterPro. GO:0008270; F:zinc ion binding; IEA:InterPro. GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW. GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IMP:UniProtKB. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |