CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012497
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial 
Protein Synonyms/Alias
 Pyruvate dehydrogenase kinase isoform 2; PDH kinase 2; PDKII 
Gene Name
 PDK2 
Gene Synonyms/Alias
 PDHK2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
376ERLPVYNKSAWRHYQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Serine/threonine kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis. 
Sequence Annotation
 DOMAIN 135 364 Histidine kinase.
 NP_BIND 251 258 ATP.
 NP_BIND 309 310 ATP (By similarity).
 NP_BIND 325 330 ATP.
 BINDING 290 290 ATP.  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Carbohydrate metabolism; Complete proteome; Glucose metabolism; Kinase; Mitochondrion; Nucleotide-binding; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 407 AA 
Protein Sequence
MRWVWALLKN ASLAGAPKYI EHFSKFSPSP LSMKQFLDFG SSNACEKTSF TFLRQELPVR 60
LANIMKEINL LPDRVLSTPS VQLVQSWYVQ SLLDIMEFLD KDPEDHRTLS QFTDALVTIR 120
NRHNDVVPTM AQGVLEYKDT YGDDPVSNQN IQYFLDRFYL SRISIRMLIN QHTLIFDGST 180
NPAHPKHIGS IDPNCNVSEV VKDAYDMAKL LCDKYYMASP DLEIQEINAA NSKQPIHMVY 240
VPSHLYHMLF ELFKNAMRAT VESHESSLIL PPIKVMVALG EEDLSIKMSD RGGGVPLRKI 300
ERLFSYMYST APTPQPGTGG TPLAGFGYGL PISRLYAKYF QGDLQLFSME GFGTDAVIYL 360
KALSTDSVER LPVYNKSAWR HYQTIQEAGD WCVPSTEPKN TSTYRVS 407 
Gene Ontology
 GO:0005967; C:mitochondrial pyruvate dehydrogenase complex; ISS:UniProtKB.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0004740; F:pyruvate dehydrogenase (acetyl-transferring) kinase activity; IDA:UniProtKB.
 GO:0031670; P:cellular response to nutrient; ISS:UniProtKB.
 GO:0034614; P:cellular response to reactive oxygen species; IMP:UniProtKB.
 GO:0042593; P:glucose homeostasis; ISS:UniProtKB.
 GO:0006006; P:glucose metabolic process; TAS:ProtInc.
 GO:0008286; P:insulin receptor signaling pathway; ISS:UniProtKB.
 GO:0072332; P:intrinsic apoptotic signaling pathway by p53 class mediator; IMP:UniProtKB.
 GO:0006090; P:pyruvate metabolic process; TAS:Reactome.
 GO:0010510; P:regulation of acetyl-CoA biosynthetic process from pyruvate; ISS:UniProtKB.
 GO:0006111; P:regulation of gluconeogenesis; ISS:UniProtKB.
 GO:0006885; P:regulation of pH; ISS:UniProtKB. 
Interpro
 IPR018955; BCDHK/PDK_N.
 IPR003594; HATPase_ATP-bd.
 IPR005467; Sig_transdc_His_kinase_core. 
Pfam
 PF10436; BCDHK_Adom3
 PF02518; HATPase_c 
SMART
 SM00387; HATPase_c 
PROSITE
 PS50109; HIS_KIN 
PRINTS