UniProt Accession

 DLDH_RAT; Q6P6R2 

Genbank Protein ID

 BC062069 

Genbank Nucleotide ID

 AAH62069.1 

Protein Name

 Dihydrolipoyl dehydrogenase, mitochondrial 

Protein Synonyms/Alias

 Dihydrolipoamide dehydrogenase 

Gene Name

 Dld 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
104	YYHLAHGKDFASRGI	acetylation	[1]
132	EQKRSAVKALTGGIA	acetylation	[1]
143	GGIAHLFKQNKVVHV	acetylation	[1]
159	GFGKITGKNQVTATT	acetylation	[1]
267	NFQRILQKQGFKFKL	acetylation	[1]
273	QKQGFKFKLNTKVTG	acetylation	[1]
277	FKFKLNTKVTGATKK	acetylation	[1]
334	LGIELDPKGRIPVNT	acetylation	[1]
346	VNTRFQTKIPNIFAI	acetylation	[1]
440	ADTDGMVKILGHKST	acetylation	[1]
445	MVKILGHKSTDRILG	acetylation	[1]
505	NLAASFGKPINF***	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction (By similarity). 

Sequence Annotation

 NP_BIND 71 80 FAD (By similarity).
 NP_BIND 183 185 FAD (By similarity).
 NP_BIND 220 227 NAD (By similarity).
 NP_BIND 361 364 FAD (By similarity).
 ACT_SITE 487 487 Proton acceptor (By similarity).
 BINDING 89 89 FAD (By similarity).
 BINDING 154 154 FAD; via amide nitrogen and carbonyl
 BINDING 243 243 NAD (By similarity).
 BINDING 278 278 NAD; via amide nitrogen and carbonyl
 BINDING 314 314 NAD; via amide nitrogen (By similarity).
 BINDING 355 355 FAD (By similarity).
 MOD_RES 127 127 N6-acetyllysine (By similarity).
 MOD_RES 143 143 N6-acetyllysine (By similarity).
 MOD_RES 410 410 N6-acetyllysine (By similarity).
 MOD_RES 417 417 N6-acetyllysine (By similarity).
 DISULFID 80 85 Redox-active (By similarity).  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Disulfide bond; FAD; Flavoprotein; Mitochondrion; NAD; Oxidoreductase; Phosphoprotein; Redox-active center; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 509 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:RGD.
 GO:0045252; C:oxoglutarate dehydrogenase complex; IDA:RGD.
 GO:0045254; C:pyruvate dehydrogenase complex; IDA:RGD.
 GO:0004148; F:dihydrolipoyl dehydrogenase activity; IDA:RGD.
 GO:0050660; F:flavin adenine dinucleotide binding; IDA:RGD.
 GO:0043544; F:lipoamide binding; IDA:RGD.
 GO:0051287; F:NAD binding; IDA:RGD.
 GO:0006103; P:2-oxoglutarate metabolic process; IDA:RGD.
 GO:0006086; P:acetyl-CoA biosynthetic process from pyruvate; IDA:RGD.
 GO:0007568; P:aging; IEP:RGD.
 GO:0045454; P:cell redox homeostasis; IEA:InterPro.
 GO:0051068; P:dihydrolipoamide metabolic process; IDA:RGD.
 GO:0009106; P:lipoate metabolic process; IDA:RGD. 

Interpro

 IPR016156; FAD/NAD-linked_Rdtase_dimer.
 IPR013027; FAD_pyr_nucl-diS_OxRdtase.
 IPR006258; Lipoamide_DH.
 IPR004099; Pyr_nucl-diS_OxRdtase_dimer.
 IPR023753; Pyr_nucl-diS_OxRdtase_FAD/NAD.
 IPR012999; Pyr_OxRdtase_I_AS.
 IPR001327; Pyr_OxRdtase_NAD-bd_dom. 

Pfam

 PF00070; Pyr_redox
 PF07992; Pyr_redox_2
 PF02852; Pyr_redox_dim 

SMART

  

PROSITE

 PS00076; PYRIDINE_REDOX_1 

PRINTS

 PR00368; FADPNR.