UniProt Accession

 VPS36_MOUSE; Q91XD6; Q9CVA3 

Genbank Protein ID

 BC010811; AK008946 

Genbank Nucleotide ID

 AAH10811.1; BAB25984.1 

Protein Name

 Vacuolar protein-sorting-associated protein 36 

Protein Synonyms/Alias

 ESCRT-II complex subunit VPS36 

Gene Name

 Vps36 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
34	YDGEEKIKFDAGTLL	acetylation	[1]
144	SQSLQTNKGPQPGRV	ubiquitination	[2]
189	SKLMIKAKEMVELSK	ubiquitination	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3 (By similarity). 

Sequence Annotation

 DOMAIN 1 88 GLUE N-terminal.
 DOMAIN 105 138 GLUE C-terminal.  

Keyword

 3D-structure; Coiled coil; Complete proteome; Cytoplasm; Endosome; Lipid-binding; Membrane; Nucleus; Protein transport; Reference proteome; Transcription; Transcription regulation; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 386 AA 

Protein Sequence

Gene Ontology

 GO:0005770; C:late endosome; IDA:MGI.
 GO:0005764; C:lysosome; IDA:MGI.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0032266; F:phosphatidylinositol-3-phosphate binding; IEA:InterPro.
 GO:0043130; F:ubiquitin binding; IDA:MGI.
 GO:0016197; P:endosomal transport; IC:MGI.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR007286; EAP30.
 IPR021648; VPS36_GLUE.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF04157; EAP30
 PF11605; Vps36_ESCRT-II 

SMART

  

PROSITE

 PS51495; GLUE 

PRINTS