UniProt Accession

 CPSF5_RAT; Q4KM65 

Genbank Protein ID

 BC098748 

Genbank Nucleotide ID

 AAH98748.1 

Protein Name

 Cleavage and polyadenylation specificity factor subunit 5 

Protein Synonyms/Alias

 Nucleoside diphosphate-linked moiety X motif 21; Nudix motif 21 

Gene Name

 Nudt21 

Gene Synonyms/Alias

 Cpsf5 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
23	GVNQFGNKYIQQTKP	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export (By similarity). 

Sequence Annotation

 DOMAIN 76 201 Nudix hydrolase.
 REGION 2 147 Necessary for RNA-binding (By
 REGION 81 160 Necessary for interactions with PAPOLA
 REGION 102 104 Interaction with RNA (By similarity).
 MOTIF 109 130 Nudix box.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 23 23 N6-acetyllysine (By similarity).
 MOD_RES 29 29 N6-acetyllysine (By similarity).
 MOD_RES 40 40 Phosphotyrosine (By similarity).
 MOD_RES 56 56 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; mRNA processing; Nucleus; Phosphoprotein; Reference proteome; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 227 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IEA:Compara.
 GO:0005849; C:mRNA cleavage factor complex; ISS:UniProtKB.
 GO:0042382; C:paraspeckles; ISS:UniProtKB.
 GO:0017091; F:AU-rich element binding; ISS:UniProtKB.
 GO:0016787; F:hydrolase activity; IEA:InterPro.
 GO:0003729; F:mRNA binding; ISS:UniProtKB.
 GO:0006378; P:mRNA polyadenylation; ISS:UniProtKB.
 GO:0051262; P:protein tetramerization; IEA:Compara. 

Interpro

 IPR016706; Cleav_polyA_spec_factor_su5.
 IPR000086; NUDIX_hydrolase_dom.
 IPR015797; NUDIX_hydrolase_dom-like. 

Pfam

 PF13869; NUDIX_2 

SMART

  

PROSITE

 PS51462; NUDIX
 PS00893; NUDIX_BOX 

PRINTS