CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021063
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphoinositide 3-kinase adapter protein 1 
Protein Synonyms/Alias
 B-cell adapter for phosphoinositide 3-kinase; B-cell phosphoinositide 3-kinase adapter protein 1 
Gene Name
 Pik3ap1 
Gene Synonyms/Alias
 Bcap 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
403VETVDMLKTHIKEELubiquitination[1]
488TKDSMIRKFLEGNSVubiquitination[1]
496FLEGNSVKPASWEREubiquitination[1]
561ISKVFAEKSQERLGNubiquitination[1]
601YDPFAGMKTPGQRQLubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Signaling adapter that contributes to B-cell development by linking B-cell receptor (BCR) signaling to the phosphoinositide 3-kinase (PI3K)-Akt signaling pathway. Has a complementary role to the BCR coreceptor CD19, coupling BCR and PI3K activation by providing a docking site for the PI3K subunit PIK3R1. Alternatively, links Toll-like receptor (TLR) signaling to PI3K activation, a process preventing excessive inflammatory cytokine production. Also involved in the activation of PI3K in natural killer cells. May be involved in the survival of mature B-cells via activation of REL. 
Sequence Annotation
 DOMAIN 182 318 DBB.
 REGION 10 145 Necessary and sufficent to mediate
 MOD_RES 264 264 Phosphotyrosine.
 MOD_RES 420 420 Phosphotyrosine; by SYK (Probable).
 MOD_RES 445 445 Phosphotyrosine; by SYK (Probable).
 MOD_RES 460 460 Phosphotyrosine; by SYK (Probable).
 MOD_RES 513 513 Phosphotyrosine; by ABL1 (By similarity).
 MOD_RES 553 553 Phosphotyrosine; by ABL1 (By similarity).
 MOD_RES 570 570 Phosphotyrosine; by ABL1 (By similarity).
 MOD_RES 594 594 Phosphotyrosine; by ABL1 (By similarity).
 MOD_RES 694 694 Phosphotyrosine; by ABL1 (By similarity).  
Keyword
 Alternative splicing; Cell membrane; Complete proteome; Cytoplasm; Membrane; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 811 AA 
Protein Sequence
MAASGWGRGC DILIFYSPDA EEWCQYLQDL FVSCRQVRSQ KTQTYRLVPD ASFSAQDLWV 60
FRDARCVLVL LSAGLVGCFG QPGLLPMLQR ACHPPQRVVR LLCGVQPGDE DFQAFFPDWA 120
HWQEMTCDDE PETYLAAVRK AISEDSGCDS VTDTEPEDER ELPFSKQTNL PPEISPGNLM 180
VVQPDRIRCG AETTVYIIVR CKLDEKVSTE AEFSPEDSPS IRVEGTLENE YTVSVKAPDL 240
SSGNVSLKVY SGDLVVCETT VSYYTDMEEI GNLLSSAANP VEFMCQAFKI VPYNTETLDK 300
LLTESLKNNI PASGLHLFGI NQLEEDDMMT NQRDEELPTL LHFAAKYGLK NLTALLLTCP 360
GALQAYSVAN KHGHYPNTIA EKHGFRDLRQ FIDEYVETVD MLKTHIKEEL MQGEEADDVY 420
ESMAHLSTDL LMKCSLNPGC DDELYESMAA FAPAATEDLY VEMLQASAGN PVSGESFSRP 480
TKDSMIRKFL EGNSVKPASW EREQHHPYGE ELYHIVDEDE TFSVDLANRP PVPVPRPEAS 540
APGPPPPPDN EPYISKVFAE KSQERLGNFY VSSESIRKEP LVRPWRDRPP SSIYDPFAGM 600
KTPGQRQLIT LQEQVKLGIV NVDEAVLHFK EWQLNQKKRS ESFRFQQENL KRLRESITRR 660
RKEKPKSGKH TDLEITVPIR HSQHLPEKVE FGVYESGPRK SVLPARTELR RGDWKTDSMS 720
STASSTSNRS STRSLLSVSS GMEGDNEDNE IPEITRSRGP GPTQVDGAPV VTGTPVGTLE 780
RPPRVPPRAA SQRPLTRESF HPPPPVPPRG R 811 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:MGI.
 GO:0036312; F:phosphatidylinositol 3-kinase regulatory subunit binding; IDA:UniProtKB.
 GO:0034122; P:negative regulation of toll-like receptor signaling pathway; IMP:UniProtKB.
 GO:0014068; P:positive regulation of phosphatidylinositol 3-kinase cascade; IMP:UniProtKB.
 GO:0034123; P:positive regulation of toll-like receptor signaling pathway; IMP:UniProtKB.
 GO:0050727; P:regulation of inflammatory response; IMP:UniProtKB.
 GO:0034134; P:toll-like receptor 2 signaling pathway; IMP:UniProtKB.
 GO:0034142; P:toll-like receptor 4 signaling pathway; IMP:UniProtKB.
 GO:0034154; P:toll-like receptor 7 signaling pathway; IMP:UniProtKB.
 GO:0034162; P:toll-like receptor 9 signaling pathway; IMP:UniProtKB. 
Interpro
 IPR017893; DBB_domain. 
Pfam
  
SMART
  
PROSITE
 PS51376; DBB 
PRINTS