CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023279
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein SCAF8 
Protein Synonyms/Alias
 CDC5L complex-associated protein 7; RNA-binding motif protein 16; SR-related and CTD-associated factor 8 
Gene Name
 SCAF8 
Gene Synonyms/Alias
 CCAP7; KIAA1116; RBM16 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
18LYSLNDYKPPISKAKsumoylation[1]
119RVLNLWQKNNVFKSEubiquitination[2]
Reference
 [1] In vivo identification of sumoylation sites by a signature tag and cysteine-targeted affinity purification.
 Blomster HA, Imanishi SY, Siimes J, Kastu J, Morrice NA, Eriksson JE, Sistonen L.
 J Biol Chem. 2010 Jun 18;285(25):19324-9. [PMID: 20388717]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 May play a role in mRNA processing. 
Sequence Annotation
 DOMAIN 1 139 CID.
 DOMAIN 477 551 RRM.
 MOD_RES 615 615 Phosphothreonine.
 MOD_RES 617 617 Phosphoserine.
 CROSSLNK 18 18 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Complete proteome; Isopeptide bond; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; RNA-binding; Spliceosome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1271 AA 
Protein Sequence
MEAVKTFNSE LYSLNDYKPP ISKAKMTQIT KAAIKAIKFY KHVVQSVEKF IQKCKPEYKV 60
PGLYVIDSIV RQSRHQFGQE KDVFAPRFSN NIISTFQNLY RCPGDDKSKI VRVLNLWQKN 120
NVFKSEIIQP LLDMAAGIPP PVVTPVLAST TTAMSNTPGT PVTPVTPANV VQGLPDPWVS 180
QITNTDTLAA VAQILQSPQG QQLQQLIQTL QIQQQKPQPS ILQALDAGLV VQLQALTAQL 240
TAAAAAANTL TPLEQGVSFN KKLMDRFDFG EDSEHSEEPK KEIPASQLSH VSESVNNSIF 300
HQIAEQLQQQ NLEHLRQQLL EQQQPQKATP QDSQEGTFGS EHSASPSQGS SQQHFLEPEV 360
NLDDSIDIQQ QDMDIDEGQD GVEEEVFEQE AKKVAVRSRS RTHSRSRSRS PRKRRSRSRS 420
GSRKRKHRKR SRSRSRERKR KSSRSYSSER RAREREKERQ KKGLPPIRSK TLSVCSTTLW 480
VGQVDKKATQ QDLTNLFEEF GQIESINMIP PRGCAYVCMV HRQDAFRALQ KLSSGSYKIG 540
SKVIKIAWAL NKGVKTEYKQ FWDVDLGVTY IPWEKVKVDD LEGFAEGGMI DQETVNTEWE 600
TVKSSEPVKE TVQTTQSPTP VEKETVVTTQ AEVFPPPVAM LQIPVAPAVP TVSLVPPAFP 660
VSMPVPPPGF SPIPPPPFLR ASFNPSQPPP GFMPPPVPPP VVPPPTIPPV VPTSLVQPSL 720
SMTPETVKDV GFGSLVIPGG SVASNLATSA LPAGNVFNAP TKQAEPEEKV PHLIDHQISS 780
GENTRSVIPN DISSNAAILG GQPPNVTSNS GILGVQRPNV SSNSEILGVR PSNVSSSSGI 840
IAAQPPNILN NSGILGIQPP SVSNSSGLLG VLPPNIPNNS GLVGVQPPNV PNTPGLLGTQ 900
PPAGPQNLPP LSIPNQRMPT MPMLDIRPGL IPQAPGPRFP LIQPGIPPQR GIPPPSVLDS 960
ALHPPPRGPF PPGDIFSQPE RPFLAPGRQS VDNVTNPEKR IPLGNDNIQQ EGDRDYRFPP 1020
IETRESISRP PPVDVRDVVG RPIDPREGPG RPPLDGRDHF GRPPVDIREN LVRPGIDHLG 1080
RRDHFGFNPE KPWGHRGDFD EREHRVLPVY GGPKGLHEER GRFRSGNYRF DPRSGPWNRG 1140
FGQEVHRDFD DRRRPWERQR DRDDRDFDFC REMNGNRLGR DRIQNTWVPP PHARVFDYFE 1200
GATSQRKGDN VPQVNGENTE RHAQPPPIPV QNDPELYEKL TSSNEINKEK SDTVADIESE 1260
PVVESTETEG T 1271 
Gene Ontology
 GO:0016363; C:nuclear matrix; ISS:UniProtKB.
 GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 
Interpro
 IPR006569; CID_dom.
 IPR008942; ENTH_VHS.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR006903; RNA_pol_II-bd.
 IPR000504; RRM_dom. 
Pfam
 PF04818; CTD_bind
 PF00076; RRM_1 
SMART
 SM00582; RPR
 SM00360; RRM 
PROSITE
 PS51391; CID
 PS50102; RRM 
PRINTS