CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003942
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Plasma membrane calcium-transporting ATPase 2 
Protein Synonyms/Alias
 PMCA2; Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium pump isoform 2 
Gene Name
 Atp2b2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
12TNSDFYSKNQRNESSubiquitination[1]
45RGTEAVVKIKETYGDubiquitination[1]
61ESICRRLKTSPVEGLubiquitination[1]
77GTAPDLEKRKQIFGQubiquitination[1]
191SRIEQEQKFTVVRAGubiquitination[1]
254QVRKSVDKDPMLLSGubiquitination[1]
349DSSQSKAKQQDGAAAubiquitination[1]
363AMEMQPLKSAEGGDAubiquitination[1]
522YVGDVHYKEIPDPSSubiquitination[1]
557TKILPPEKEGALPRQubiquitination[1]
595RSQMPEEKLYKVYTFubiquitination[1]
770NRRIRNEKGEIEQERubiquitination[1]
780IEQERIDKIWPKLRVubiquitination[1]
829TNDGPALKKADVGFAubiquitination[1]
830NDGPALKKADVGFAMubiquitination[1]
944RKPYGRNKPLISRTMubiquitination[1]
1101EAGRLTQKEEIPEEEubiquitination[1]
1157SLYEGLEKPESRTSIubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. 
Sequence Annotation
 REGION 1123 1140 Calmodulin-binding subdomain A (By
 REGION 1141 1150 Calmodulin-binding subdomain B (By
 ACT_SITE 499 499 4-aspartylphosphate intermediate.
 METAL 820 820 Magnesium (By similarity).
 METAL 824 824 Magnesium (By similarity).
 MOD_RES 1139 1139 Phosphothreonine; by PKC (By similarity).
 MOD_RES 1201 1201 Phosphoserine; by PKA (By similarity).
 MOD_RES 1231 1231 Phosphoserine (By similarity).
 MOD_RES 1234 1234 Phosphoserine (By similarity).
 MOD_RES 1238 1238 Phosphoserine (By similarity).
 MOD_RES 1241 1241 Phosphothreonine (By similarity).
 MOD_RES 1242 1242 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; ATP-binding; Calcium; Calcium transport; Calmodulin-binding; Cell membrane; Complete proteome; Hydrolase; Ion transport; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1243 AA 
Protein Sequence
MGDMTNSDFY SKNQRNESSH GGEFGCSMEE LRSLMELRGT EAVVKIKETY GDTESICRRL 60
KTSPVEGLPG TAPDLEKRKQ IFGQNFIPPK KPKTFLQLVW EALQDVTLII LEIAAIISLG 120
LSFYHPPGES NEGCATAQGG AEDEGEAEAG WIEGAAILLS VICVVLVTAF NDWSKEKQFR 180
GLQSRIEQEQ KFTVVRAGQV VQIPVAEIVV GDIAQIKYGD LLPADGLFIQ GNDLKIDESS 240
LTGESDQVRK SVDKDPMLLS GTHVMEGSGR MVVTAVGVNS QTGIIFTLLG AGGEEEEKKD 300
KKGVKKGDGL QLPAADGAAP ANAAGSANAS LVNGKMQDGS ADSSQSKAKQ QDGAAAMEMQ 360
PLKSAEGGDA DDKKKANMHK KEKSVLQGKL TKLAVQIGKA GLVMSAITVI ILVLYFTVDT 420
FVVNKKPWLT ECTPVYVQYF VKFFIIGVTV LVVAVPEGLP LAVTISLAYS VKKMMKDNNL 480
VRHLDACETM GNATAICSDK TGTLTTNRMT VVQAYVGDVH YKEIPDPSSI NAKTLELLVN 540
AIAINSAYTT KILPPEKEGA LPRQVGNKTE CGLLGFVLDL RQDYEPVRSQ MPEEKLYKVY 600
TFNSVRKSMS TVIKMPDESF RMYSKGASEI VLKKCCKILS GAGEPRVFRP RDRDEMVKKV 660
IEPMACDGLR TICVAYRDFP SSPEPDWDNE NDILNELTCI CVVGIEDPVR PEVPEAIRKC 720
QRAGITVRMV TGDNINTARA IAIKCGIIHP GEDFLCLEGK EFNRRIRNEK GEIEQERIDK 780
IWPKLRVLAR SSPTDKHTLV KGIIDSTHTE QRQVVAVTGD GTNDGPALKK ADVGFAMGIA 840
GTDVAKEASD IILTDDNFSS IVKAVMWGRN VYDSISKFLQ FQLTVNVVAV IVAFTGACIT 900
QDSPLKAVQM LWVNLIMDTF ASLALATEPP TETLLLRKPY GRNKPLISRT MMKNILGHAV 960
YQLTLIFTLL FVGEKMFQID SGRNAPLHSP PSEHYTIIFN TFVMMQLFNE INARKIHGER 1020
NVFDGIFRNP IFCTIVLGTF AIQIVIVQFG GKPFSCSPLQ LDQWMWCIFI GLGELVWGQV 1080
IATIPTSRLK FLKEAGRLTQ KEEIPEEELN EDVEEIDHAE RELRRGQILW FRGLNRIQTQ 1140
IRVVKAFRSS LYEGLEKPES RTSIHNFMAH PEFRIEDSQP HIPLIDDTDL EEDAALKQNS 1200
SPPSSLNKNN SAIDSGINLT TDTSKSATSS SPGSPIHSLE TSL 1243 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IDA:RGD.
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0030425; C:dendrite; IDA:RGD.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0045121; C:membrane raft; IDA:RGD.
 GO:0032809; C:neuronal cell body membrane; IDA:RGD.
 GO:0005886; C:plasma membrane; ISS:UniProtKB.
 GO:0033130; F:acetylcholine receptor binding; IDA:RGD.
 GO:0005524; F:ATP binding; IDA:RGD.
 GO:0005509; F:calcium ion binding; ISS:UniProtKB.
 GO:0005388; F:calcium-transporting ATPase activity; IDA:RGD.
 GO:0005516; F:calmodulin binding; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0030165; F:PDZ domain binding; ISS:UniProtKB.
 GO:0008022; F:protein C-terminus binding; ISS:UniProtKB.
 GO:0007420; P:brain development; IEP:RGD.
 GO:1901660; P:calcium ion export; IMP:RGD.
 GO:0003407; P:neural retina development; IEP:RGD.
 GO:0030182; P:neuron differentiation; ISS:UniProtKB.
 GO:0007605; P:sensory perception of sound; ISS:UniProtKB. 
Interpro
 IPR022141; ATP_Ca_trans_C.
 IPR006408; ATPase_P-typ_Ca-transp_plasma.
 IPR006068; ATPase_P-typ_cation-transptr_C.
 IPR004014; ATPase_P-typ_cation-transptr_N.
 IPR023299; ATPase_P-typ_cyto_domN.
 IPR018303; ATPase_P-typ_P_site.
 IPR023298; ATPase_P-typ_TM_dom.
 IPR008250; ATPase_P-typ_transduc_dom_A.
 IPR001757; Cation_transp_P_typ_ATPase.
 IPR023214; HAD-like_dom. 
Pfam
 PF12424; ATP_Ca_trans_C
 PF00689; Cation_ATPase_C
 PF00690; Cation_ATPase_N
 PF00122; E1-E2_ATPase
 PF00702; Hydrolase 
SMART
 SM00831; Cation_ATPase_N 
PROSITE
 PS00154; ATPASE_E1_E2 
PRINTS
 PR00119; CATATPASE.