CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017647
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Trinucleotide repeat-containing gene 6A protein 
Protein Synonyms/Alias
 CAG repeat protein 26; EMSY interactor protein; GW182 autoantigen; Protein GW1; Glycine-tryptophan protein of 182 kDa 
Gene Name
 TNRC6A 
Gene Synonyms/Alias
 CAGH26; KIAA1460; TNRC6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
1741PPGLTGQKPPLSTWDubiquitination[1]
1786ITNWLVLKNLTPQIDubiquitination[1]
1826ALVRYSSKEEVVKAQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA- dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. As scaffoldng protein associates with argonaute proteins bound to partially complementary mRNAs and simultaneously can recruit CCR4-NOT and PAN deadenylase complexes. 
Sequence Annotation
 DOMAIN 1781 1853 RRM.
 REGION 1 932 Interaction with argonaute family
 REGION 254 503 Sufficient for interaction with AGO1,
 REGION 270 346 Sufficient for interaction with AGO2.
 REGION 318 399 Sufficient for interaction with AGO2.
 REGION 340 439 Sufficient for interaction with AGO2.
 REGION 409 495 Sufficient for interaction with AGO2.
 REGION 502 751 Sufficient for interaction with AGO2.
 REGION 566 1343 Sufficient for interaction with AGO1 and
 REGION 1074 1144 Sufficient for interaction with AGO2.
 REGION 1604 1622 PABPC1-interacting motif-2 (PAM2).
 REGION 1670 1962 Sufficient for interaction with AGO2.
 MOD_RES 991 991 Phosphoserine (By similarity).
 MOD_RES 1470 1470 Phosphothreonine.
 MOD_RES 1585 1585 Phosphoserine.
 MOD_RES 1869 1869 Phosphoserine.  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Phosphoprotein; Polymorphism; Reference proteome; RNA-binding; RNA-mediated gene silencing; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1962 AA 
Protein Sequence
MRELEAKATK DVERNLSRDL VQEEEQLMEE KKKKKDDKKK KEAAQKKATE QKIKVPEQIK 60
PSVSQPQPAN SNNGTSTATS TNNNAKRATA NNQQPQQQQQ QQQPQQQQPQ QQPQPQPQQQ 120
QPQQQPQALP RYPREVPPRF RHQEHKQLLK RGQHFPVIAA NLGSAVKVLN SQSESSALTN 180
QQPQNNGEVQ NSKNQSDINH STSGSHYENS QRGPVSSTSD SSTNCKNAVV SDLSEKEAWP 240
SAPGSDPELA SECMDADSAS SSESERNITI MASGNTGGEK DGLRNSTGLG SQNKFVVGSS 300
SNNVGHGSST GPWGFSHGAI ISTCQVSVDA PESKSESSNN RMNAWGTVSS SSNGGLNPST 360
LNSASNHGAW PVLENNGLAL KGPVGSGSSG INIQCSTIGQ MPNNQSINSK VSGGSTHGTW 420
GSLQETCESE VSGTQKVSFS GQPQNITTEM TGPNNTTNFM TSSLPNSGSV QNNELPSSNT 480
GAWRVSTMNH PQMQAPSGMN GTSLSHLSNG ESKSGGSYGT TWGAYGSNYS GDKCSGPNGQ 540
ANGDTVNATL MQPGVNGPMG TNFQVNTNKG GGVWESGAAN SQSTSWGSGN GANSGGSRRG 600
WGTPAQNTGT NLPSVEWNKL PSNQHSNDSA NGNGKTFTNG WKSTEEEDQG SATSQTNEQS 660
SVWAKTGGTV ESDGSTESTG RLEEKGTGES QSRDRRKIDQ HTLLQSIVNR TDLDPRVLSN 720
SGWGQTPIKQ NTAWDTETSP RGERKTDNGT EAWGSSATQT FNSGACIDKT SPNGNDTSSV 780
SGWGDPKPAL RWGDSKGSNC QGGWEDDSAA TGMVKSNQWG NCKEEKAAWN DSQKNKQGWG 840
DGQKSSQGWS VSASDNWGET SRNNHWGEAN KKSSSGGSDS DRSVSGWNEL GKTSSFTWGN 900
NINPNNSSGW DESSKPTPSQ GWGDPPKSNQ SLGWGDSSKP VSSPDWNKQQ DIVGSWGIPP 960
ATGKPPGTGW LGGPIPAPAK EEEPTGWEEP SPESIRRKME IDDGTSAWGD PSKYNYKNVN 1020
MWNKNVPNGN SRSDQQAQVH QLLTPASAIS NKEASSGSGW GEPWGEPSTP ATTVDNGTSA 1080
WGKPIDSGPS WGEPIAAASS TSTWGSSSVG PQALSKSGPK SMQDGWCGDD MPLPGNRPTG 1140
WEEEEDVEIG MWNSNSSQEL NSSLNWPPYT KKMSSKGLSG KKRRRERGMM KGGNKQEEAW 1200
INPFVKQFSN ISFSRDSPEE NVQSNKMDLS GGMLQDKRME IDKHSLNIGD YNRTVGKGPG 1260
SRPQISKESS MERNPYFDKD GIVADESQNM QFMSSQSMKL PPSNSALPNQ ALGSIAGLGM 1320
QNLNSVRQNG NPSMFGVGNT AAQPRGMQQP PAQPLSSSQP NLRAQVPPPL LSPQVPVSLL 1380
KYAPNNGGLN PLFGPQQVAM LNQLSQLNQL SQISQLQRLL AQQQRAQSQR SVPSGNRPQQ 1440
DQQGRPLSVQ QQMMQQSRQL DPNLLVKQQT PPSQQQPLHQ PAMKSFLDNV MPHTTPELQK 1500
GPSPINAFSN FPIGLNSNLN VNMDMNSIKE PQSRLRKWTT VDSISVNTSL DQNSSKHGAI 1560
SSGFRLEESP FVPYDFMNSS TSPASPPGSI GDGWPRAKSP NGSSSVNWPP EFRPGEPWKG 1620
YPNIDPETDP YVTPGSVINN LSINTVREVD HLRDRNSGSS SSLNTTLPST SAWSSIRASN 1680
YNVPLSSTAQ STSARNSDSK LTWSPGSVTN TSLAHELWKV PLPPKNITAP SRPPPGLTGQ 1740
KPPLSTWDNS PLRIGGGWGN SDARYTPGSS WGESSSGRIT NWLVLKNLTP QIDGSTLRTL 1800
CMQHGPLITF HLNLPHGNAL VRYSSKEEVV KAQKSLHMCV LGNTTILAEF ASEEEISRFF 1860
AQSQSLTPSP GWQSLGSSQS RLGSLDCSHS FSSRTDLNHW NGAGLSGTNC GDLHGTSLWG 1920
TPHYSTSLWG PPSSSDPRGI SSPSPINAFL SVDHLGGGGE SM 1962 
Gene Ontology
 GO:0000932; C:cytoplasmic mRNA processing body; IDA:MGI.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0035068; C:micro-ribonucleoprotein complex; IDA:UniProtKB.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0009267; P:cellular response to starvation; IEA:Compara.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0010467; P:gene expression; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0035278; P:negative regulation of translation involved in gene silencing by miRNA; IDA:UniProtKB.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0007219; P:Notch signaling pathway; TAS:Reactome.
 GO:0048015; P:phosphatidylinositol-mediated signaling; TAS:Reactome. 
Interpro
 IPR019486; Argonaute_hook_dom.
 IPR026804; GW182_fam.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom.
 IPR009060; UBA-like. 
Pfam
 PF10427; Ago_hook 
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS